Tomasz Lozinski
Polish Academy of Sciences
7 Papers
22 Citations
Tomasz Lozinski is an academic researcher from Polish Academy of Sciences. The author has contributed to research in topics: Promoter & RNA polymerase. The author has an hindex of 6, co-authored 7 publications.
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Papers
Dissecting the functional program of Escherichia coli promoters: the combined mode of action of Lac repressor and AraC activator.
TL;DR: A three step model of transcription initiation which reveals mechanisms of AraC and LacR action is yielded which shows three distinct rate limiting steps at which AraC can exert its function.
114
Effect of DNA bending in various regions of a consensuslike Escherichia coli promoter on its strength in vivo and structure of the open complex in vitro
TL;DR: The strength of these promoters measured in vivo in relation to an internal transcriptional standard was shown to be significantly lower than that of the group without the T5(-34...-38) tract, and the promoters with two T6 tracts in the spacer had lower transcriptional activity.
32
Effect of reversed orientation and length of An.Tn DNA bending sequences in the -35 and spacer domains of a consensus-like Escherichia coli promoter on its strength in vivo and gross structure of the open complex in vitro.
Tomasz Lozinski,K L Wierzchowski +1 more
TL;DR: Results obtained show that bending of the DNA helix axis immediately upstream of the -35 domain generally lowers the promoter strength in vivo and brings about shortening of the mean square end-to-end distance between free DNA ends in the open complex in vitro.
Effect of Mg2+ on kinetics of oxidation of pyrimidines in duplex DNA by potassium permanganate.
Tomasz Lozinski,K L Wierzchowski +1 more
TL;DR: Comparison of kj data for corresponding Ts located between +1 and -10 regions of Pa promoter in dsDNA and in ssDNA form in the open transcription complex, reported elsewhere, demonstrates that reactivity of pyrimidines indsDNA is by 2-3 orders of magnitude smaller.
8
Effect of A(n) tracts within the UP element proximal subsite of a model promoter on kinetics of open complex formation by Escherichia coli RNA polymerase.
TL;DR: Kinetics of the formation of RPo by Escherichia coli RNA polymerase at two groups of synthetic consensus-like promoters bearing single DNA bending tracts provide a new insight to and support the present model of interactions betweenRNA polymerase alpha and sigma(70) subunits with the proximal UP subsite and the -35 region of promoters.