Tomas Strucko
Technical University of Denmark
19 Papers
6 Citations
Tomas Strucko is an academic researcher from Technical University of Denmark. The author has contributed to research in topics: Biology & Saccharomyces cerevisiae. The author has an hindex of 6, co-authored 14 publications. Previous affiliations of Tomas Strucko include Novo Nordisk Foundation.
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Papers
EasyClone: method for iterative chromosomal integration of multiple genes in Saccharomyces cerevisiae
Niels Bjerg Jensen,Tomas Strucko,Kanchana Rueksomtawin Kildegaard,Florian David,Jerome Maury,Uffe Hasbro Mortensen,Jochen Förster,Jens Nielsen,Jens Nielsen,Irina Borodina +9 more
TL;DR: It is demonstrated that selective markers can be simultaneously removed using Cre-mediated recombination and all the integrated heterologous genes remain in the chromosome and show unchanged expression levels, suitable for metabolic engineering in yeast where multiple rounds of gene introduction and marker recycling can be carried out.
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Metabolic engineering of yeast for fermentative production of flavonoids
Angelica Rodriguez,Tomas Strucko,Steen Gustav Stahlhut,Mette Kristensen,Daniel Killerup Svenssen,Jochen Förster,Jens Nielsen,Irina Borodina +7 more
TL;DR: Yeast Saccharomyces cerevisiae was engineered for de novo production of six different flavonoids directly from glucose, without supplementation of expensive intermediates, demonstrating the potential of flavonoid-producing yeast cell factories.
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Cpf1 enables fast and efficient genome editing in Aspergilli
Katherina Garcia Vanegas,Zofia Dorota Jarczynska,Tomas Strucko,Uffe Hasbro Mortensen +3 more
- 01 May 2019
TL;DR: The experiments demonstrate that Cpf1 can be efficiently used in Aspergilli for gene editing thereby expanding the range of genomic DNA sequences that can be targeted by CRISPR technologies.
A Versatile in Vivo DNA Assembly Toolbox for Fungal Strain Engineering
Zofia Dorota Jarczynska,Katherina Garcia Vanegas,Marcus Deichmann,Christina Nørskov Jensen,Marouschka J. Scheeper,Malgorzata E. Futyma,Tomas Strucko,Fabiano Jares Contesini,Tue Sparholt Jørgensen,Jakob Blæsbjerg Hoof,Uffe Hasbro Mortensen +10 more
TL;DR: A novel fungal in vivo DNA assembly toolbox for simple and flexible genetic engineering of filamentous fungi by determining the genetic- and sequence-length requirements and demonstrating that in nonhomologous end-joining deficient strains of Aspergillus nidulans, efficient fusions can be achieved by 25 bp sequence overlaps.
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DIVERSIFY: A Fungal Multispecies Gene Expression Platform.
Zofia Dorota Jarczynska,Jakob K. H. Rendsvig,Nichlas Pagels,Veronica R. Viana,Christina Spuur Nødvig,Ferdinand H. Kirchner,Tomas Strucko,Michael Lynge Nielsen,Uffe Hasbro Mortensen +8 more
TL;DR: In this paper, a flexible platform for multispecies heterologous gene expression is presented, which reduces the workload to construction of a single gene expression cassette, used to transform all DiversIFY strains in order to identify the optimal cell factory host.
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