Tom H. Eyles
John Innes Centre
7 Papers
26 Citations
Tom H. Eyles is an academic researcher from John Innes Centre. The author has contributed to research in topics: Biology & Bottromycin. The author has an hindex of 3, co-authored 6 publications. Previous affiliations of Tom H. Eyles include Norwich Research Park.
Chat about Author
Papers
Uncovering the unexplored diversity of thioamidated ribosomal peptides in Actinobacteria using the RiPPER genome mining tool.
Javier Santos-Aberturas,Govind Chandra,Luca Frattaruolo,Rodney Lacret,Thu H. Pham,Natalia M. Vior,Tom H. Eyles,Andrew W. Truman +7 more
TL;DR: RiPPER, a new tool for the family-independent identification of RiPP precursor peptides, is described and it is shown that previously undescribed RiPP gene clusters encoding YcaO and TfuA proteins are widespread in Actinobacteria and encode a highly diverse landscape of precursor peptide that are predicted to make thioamidated RiPPs.
Uncovering the unexplored diversity of thioamidated ribosomal peptides in Actinobacteria using the RiPPER genome mining tool
Javier Santos-Aberturas,Govind Chandra,Luca Frattaruolo,Rodney Lacret,Thu H. Pham,Natalia M. Vior,Tom H. Eyles,Andrew W. Truman +7 more
TL;DR: RiPPER, a new tool for the family-independent identification of RiPP precursor peptides, is described and the first rational discovery of a new family of thioamidated natural products, the thiovarsolins from Streptomyces varsoviensis is described.
61
Rapid and Robust Yeast-Mediated Pathway Refactoring Generates Multiple New Bottromycin-Related Metabolites
TL;DR: This work presents a one-step yeast-based method that enables efficient, cheap, and flexible modifications to BGCs, using the BGC for the antibiotic bottromycin, and showcases multiple modifications including refactoring, gene deletions and targeted mutations.
27
Understanding Thioamitide Biosynthesis Using Pathway Engineering and Untargeted Metabolomics
TL;DR: Engineering the thiostreptamide S4 gene cluster in a heterologous host could provide insights into its biosynthesis when coupled with untargeted metabolomics and targeted mutations of the precursor peptide, paving the way for future RiPP discovery and engineering.
Regulation of Bottromycin Biosynthesis Involves an Internal Transcriptional Start Site and a Cluster-Situated Modulator
TL;DR: Insight is provided into how this deceptively complex pathway is regulated in the absence of a pathway specific master regulator, and how it might coordinate with the central metabolism of the cell.