12 Papers
15 Citations
Tin Marsic is an academic researcher from King Abdullah University of Science and Technology. The author has contributed to research in topics: Loop-mediated isothermal amplification & Biology. The author has an hindex of 5, co-authored 7 publications.
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Papers
iSCAN: An RT-LAMP-coupled CRISPR-Cas12 module for rapid, sensitive detection of SARS-CoV-2.
Zahir Ali,Rashid Aman,Ahmed Mahas,Gundra Sivakrishna Rao,Muhammad Tehseen,Tin Marsic,Rahul Salunke,Amit Kumar Subudhi,Sharif Hala,Samir M. Hamdan,Arnab Pain,Fadwa S. Alofi,Afrah Alsomali,Anwar M. Hashem,Asim Khogeer,Naif A.M. Almontashiri,Malak Abedalthagafi,Norhan Hassan,Magdy M. Mahfouz +18 more
TL;DR: The iSCAN SARS-CoV-2 detection module, which exhibits the critical features of a robust molecular diagnostic device, should facilitate the effective management and control of COVID-19.
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Efficient, Rapid, and Sensitive Detection of Plant RNA Viruses With One-Pot RT-RPA-CRISPR/Cas12a Assay.
TL;DR: In this article, a simple, rapid, and efficient RT-RPA method, coupled with a CRISPR/Cas12a-based one-step detection assay, was proposed to detect plant RNA viruses.
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LAMP-Coupled CRISPR-Cas12a Module for Rapid and Sensitive Detection of Plant DNA Viruses.
TL;DR: In this article, a clustered regularly interspaced short palindromic repeats (CRISPR)-based nucleic acid diagnostic method utilizing the CRISPR-Cas12a system for detecting two geminiviruses, tomato yellow leaf curl virus (TYLCV) and tomato leaf curl New Delhi virus (ToLCNDV), which have single-stranded DNA genomes.
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Vigilant: An Engineered VirD2-Cas9 Complex for Lateral Flow Assay-Based Detection of SARS-CoV2.
TL;DR: Vigilant as discussed by the authors uses a fusion of catalytically inactive SpCas9 endonuclease and VirD2 relaxase for sensitive, specific nucleic acid detection by LFA.
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Characterization of a thermostable Cas13 enzyme for one-pot detection of SARS-CoV-2
Ahmed Mahas,Tin Marsic,Mauricio Lopez-Portillo Masson,Qiaochu Wang,Rashid Aman,Cheng Zheng,Zahir Ali,Madain Alsanea,Ahmed A. Al-Qahtani,Bernard Ghanem,Fatimah S. Alhamlan,Magdy M. Mahfouz +11 more
TL;DR: A thermostable Cas13 orthologue from Thermoclostridium caenicola (TccCas13a) is identified and characterized, which exhibits robust cis and trans catalytic activities over a broad range of temperatures from 37 to 70 °C, and was clinically validated and is suitable for multiplexed detection.