Immortalization Strategies for Mammalian Cells Culturing Animal Cells in Fluidized Bed Reactors Viral Evaluation of Animal Cell Lines Used In Biotechnology Monitoring Animal Cell Growth and Productivity by Flow Cytometry Adaptation of Mammalian Cells Grown in Serum Free MediumCytotoxicity Testing Using Cell Lines Measuring the Folding Dynamics of Recombinant Proteins Secreted from Mammalian CellsHematopoietic Cells for Cellular and Gene Therapy: I Basic Assay Techniques Measurement of Cell-Culture Glycosidase Activity Cytogenetic Characterization of Recombinant Cells Hematopoietic Cells for Cellular and Gene Therapy: II Expansion ProtocolsControl of Proteolysis in Cell Culture Measurement of Cell Death in Culture GPI-Anchored Fusion Proteins Harvesting GPI-Anchored Proteins From CHO Cells Optimizing Gene Expression in Mammalian Cells

Animal cell biotechnology: methods and protocols.

Enzymatic assays for the assessment of toxic effects of halogenated organic contaminants in water and food. A review.

Assay ready cells are cryopreserved at a highly functional state and can be used in cell-based assay without prior cultivation or cell passaging. Basically, like any other reagent, the cells are applied to the assay instantly after thawing. Introduced initially in the drug discovery process where assay ready Frozen Instant Cells help to streamline cell-based high-throughput screening campaigns, the methodology now has been accepted for a much broader scope of applications and industries. The preparation of assay ready Frozen Instant Cells is not so much a piece of magic but a combination of good cell culture practice, careful handling, and individually optimized cryopreservation protocols. Here, a standard protocol is presented, how HepG2 cells are frozen in an assay ready quality to be used as a reagent in routine cell-based assays.

Turning Cells into Reagents: The Preparation of Assay Ready Cells for Routine Use in Bioassays.

Application of a ready-to-use cell sensor for dioxins and dioxin-like compounds screening in foodstuffs.

Aquatic animals and consumers of aquatic animals are exposed to increasingly complex mixtures of known and as-yet-unknown chemicals with dioxin-like toxicities in the water cycle. Effect- and cell-based bioanalysis can cover known and yet unknown dioxin and dioxin-like compounds as well as complex mixtures thereof but need to be standardized and integrated into international guidelines for environmental testing. In an international laboratory testing (ILT) following ISO/CD 24295 as standard procedure for rat cell-based DR CALUX un-spiked and spiked extracts of drinking-, surface-, and wastewater were validated to generate precision data for the development of the full ISO-standard. We found acceptable repeatability and reproducibility ranges below 36 % by DR CALUX bioassay for the tested un-spiked and spiked water of different origins. The presence of 17 PCDD/Fs and 12 dioxin-like PCBs was also confirmed by congener-specific GC-HRMS analysis. We compared the sum of dioxin-like activity levels measured by DR CALUX bioassay (expressed in 2,3,7,8-TCDD Bioanalytical Equivalents, BEQ) with the obtained GC-HRMS chemical analysis results converted to toxic equivalents (TEQ; van den Berg et al., 2013). 

Results of an international interlaboratory study on dioxin-like activities in drinking-, river surface- and wastewater using DR CALUX bioassay.

Development and validation of a ready to use cryo-EROD assay for the standardized screening of dioxins and dioxin-like compounds in foodstuffs.

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Development and validation of a ready to use cryo-EROD assay for the standardized screening of dioxins and dioxin-like compounds in foodstuffs.