Thomas Naiser
University of Bayreuth
8 Papers
70 Citations
Thomas Naiser is an academic researcher from University of Bayreuth. The author has contributed to research in topics: Oligonucleotide & DNA microarray. The author has an hindex of 6, co-authored 8 publications.
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Papers
Impact of point-mutations on the hybridization affinity of surface-bound DNA/DNA and RNA/DNA oligonucleotide-duplexes: Comparison of single base mismatches and base bulges
TL;DR: It is shown that DNA microarrays can resolve even subtle changes in hybridization affinity for simple target mixtures and that DNA molecular dynamics – in form of zipping of the oligonucleotide duplex – to play an important role.
Optical Study of DNA Surface Hybridization Reveals DNA Surface Density as a Key Parameter for Microarray Hybridization Kinetics
TL;DR: It is observed that the free-DNA concentration decreases considerably during hybridization, and the standard Langmuir kinetics needs to be extended to take into account the change in bulk concentration.
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A versatile maskless microscope projection photolithography system and its application in light-directed fabrication of DNA microarrays
TL;DR: A maskless microscope projection lithography system (MPLS), in which photomasks have been replaced by a Digital Micromirror Device type spatial light modulator (DMD™, Texas Instruments), which may be employed in standard photolithography.
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Versatile maskless microscope projection photolithography system and its application in light-directed fabrication of DNA microarrays
TL;DR: In this article, a maskless microscope projection lithography system (MPLS) is presented, in which photomasks have been replaced by a Digital Micromirror Device type spatial light modulator (DMD™, Texas Instruments).
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•Posted Content
Hybridization to surface-bound oligonucleotide probes: Influence of point defects
TL;DR: This work investigates how various point-mutations, comprising single base mismatches, insertions and deletions, affect hybridization of DNA-DNA oligonucleotide duplexes, and observes that single base insertions next to like-bases result in considerably larger hybridization signals than insertion next to nonidentical bases.
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