Tamara Anan’eva
4 Papers
28 Citations
Tamara Anan’eva is an academic researcher. The author has contributed to research in topics: Serial dilution & Bacteroidales. The author has an hindex of 3, co-authored 4 publications.
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Papers
Interlaboratory comparison of real-time PCR protocols for quantification of general fecal indicator bacteria
Orin C. Shanks,Mano Sivaganesan,Lindsay Peed,Catherine A. Kelty,A. Denene Blackwood,Monica R. Greene,Rachel T. Noble,Rebecca N. Bushon,Erin A. Stelzer,Julie L. Kinzelman,Tamara Anan’eva,Christopher D. Sinigalliano,David Wanless,John F. Griffith,Yiping Cao,Steve B. Weisberg,V.J. Harwood,Christopher Staley,Kevin Oshima,Manju Varma,Richard A. Haugland +20 more
TL;DR: Examination of interlaboratory variability in the measurement of enterococci and Bacteroidales concentrations from standardized, spiked, and environmental sources of DNA using the Entero1a and GenBac3qPCR methods suggests that a crude lysate is sufficient for DNA isolation unless environmental samples contain substances that can inhibit qPCR amplification.
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Multi-laboratory survey of qPCR enterococci analysis method performance in U.S. coastal and inland surface waters.
Richard A. Haugland,Shawn Siefring,Manju Varma,Kevin Oshima,Mano Sivaganesan,Yiping Cao,Meredith R. Raith,John F. Griffith,Stephen B. Weisberg,Rachel T. Noble,A. Denene Blackwood,Julie L. Kinzelman,Tamara Anan’eva,Rebecca N. Bushon,Erin A. Stelzer,V.J. Harwood,Katrina V. Gordon,Christopher D. Sinigalliano +17 more
TL;DR: The methodological option that best balanced acceptable estimated target gene recoveries with method sensitivity and avoidance of underestimated enterococci densities was Method 1609 without extract dilution and using the delta-delta calculation method.
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Examination of Diurnal Variation at a Non-Sewage Impacted Beach via qPCR and Culture Based Methods
TL;DR: In this article, surface water samples were collected in the morning and afternoon (0700 and 1200) and analyzed by both IDEXX/Colilert and qPCR/BioGx SmartBeads/OmniMix HS to determine if temporal variation in E. coli was occurring (n = 29/23, culture/qPCR).