Takao Kitagawa
Yamaguchi University
62 Papers
269 Citations
Takao Kitagawa is an academic researcher from Yamaguchi University. The author has contributed to research in topics: Pancreatic cancer & Biology. The author has an hindex of 18, co-authored 48 publications.
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Papers
PCR-mediated seamless gene deletion and marker recycling in Saccharomyces cerevisiae
Rinji Akada,Takao Kitagawa,Shohei Kaneko,Daiso Toyonaga,Sachiko Ito,Yoshito Kakihara,Hisashi Hoshida,Shigeru Morimura,Akihiko Kondo,Kenji Kida +9 more
TL;DR: A novel marker recycling procedure that leaves no scar in the genome is described, which is designated seamless gene deletion and achieved via a PCR fragment that consisted of a URA3 marker attached to a 40 base repeat‐generating sequence flanked by HIS3 targeting sequences at both ends.
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PI3K inhibitor LY294002, as opposed to wortmannin, enhances AKT phosphorylation in gemcitabine-resistant pancreatic cancer cells
Yufeng Wang,Yasuhiro Kuramitsu,Byron Baron,Takao Kitagawa,Kazuhiro Tokuda,Junko Akada,Shin Ichiro Maehara,Yoshihiko Maehara,Kazuyuki Nakamura +8 more
TL;DR: The results reveal that LY 294002 displays the opposite effect on PI3K-dependent AKT phosphorylation, which maintains cell survival from the cytotoxicity introduced by LY294002 itself in GEM-resistant pancreatic cancer cells.
Construction of a β-glucosidase expression system using the multistress-tolerant yeast Issatchenkia orientalis
Takao Kitagawa,Kenro Tokuhiro,Hidehiko Sugiyama,Katsuhiro Kohda,Naoto Isono,Makoto Hisamatsu,Haruo Takahashi,Takao Imaeda +7 more
TL;DR: The results suggest that using a genetically engineered thermotolerant yeast such as I. orientalis in SSF could lead to cost reduction because less saccharification enzymes are required.
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Conversion of human colonic adenoma cells to adenocarcinoma cells through inflammation in nude mice.
Futoshi Okada,Tokuichi Kawaguchi,Hasem Habelhah,Tokushige Kobayashi,Hiroshi Tazawa,Noritoshi Takeichi,Takao Kitagawa,Masuo Hosokawa +7 more
TL;DR: In this article, a model of progression of human colonic adenoma cells using a nude mouse was established; the progression is accelerated by coimplantation of a plastic plate.
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•Journal Article
Glyoxalase I (GLO1) is Up-regulated in Pancreatic Cancerous Tissues Compared with Related Non-cancerous Tissues
Yufeng Wang,Yasuhiro Kuramitsu,Tomio Ueno,Nobuaki Suzuki,Shigefumi Yoshino,Norio Iizuka,Junko Akada,Takao Kitagawa,Masaaki Oka,Kazuyuki Nakamura +9 more
TL;DR: Western blotting demonstrated that GLO1 was significantly overexpressed in pancreatic cancerous tissues compared with adjacent non-cancerous tissues and could be a clinically useful target in the therapy of PC.
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