T. V. S. Murthy
Harvard University
56 Papers
107 Citations
T. V. S. Murthy is an academic researcher from Harvard University. The author has contributed to research in topics: Biology & Computer science. The author has an hindex of 5, co-authored 5 publications.
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Papers
High-resolution DNA-binding specificity analysis of yeast transcription factors
Cong Zhu,Kelsey J. R. P. Byers,Rachel Patton McCord,Zhenwei Shi,Michael F. Berger,Daniel E. Newburger,Katrina Saulrieta,Katrina Saulrieta,Zachary A. Smith,Zachary A. Smith,Mita V. Shah,Mita V. Shah,Mathangi Radhakrishnan,Mathangi Radhakrishnan,Anthony A. Philippakis,Yanhui Hu,Federico De Masi,Marcin Pacek,Andreas Rolfs,T. V. S. Murthy,Joshua LaBaer,Martha L. Bulyk +21 more
TL;DR: High-resolution binding profiles for 89 known and predicted yeast TFs are determined and proteins that bind the PAC (Polymerase A and C) motif (GATGAG) and regulate ribosomal RNA transcription and processing, core cellular processes that are constituent to ribosome biogenesis are discovered.
The Pseudomonas aeruginosa PA01 Gene Collection
Joshua LaBaer,Qing Qing Qiu,Anukanth Anumanthan,Wenhong Mar,Dongmei Zuo,T. V. S. Murthy,Helen Taycher,Allison E. Halleck,Eugenie Hainsworth,Stephen Lory,Leonardo Brizuela +10 more
TL;DR: A comprehensive gene collection for this organism is constructed utilizing the annotated genome of P. aeruginosa PA01 and a highly automated and laboratory information management system (LIMS)-supported production line, which serves as a model for building clone sets for other bacteria.
Bacterial cell-free system for high-throughput protein expression and a comparative analysis of Escherichia coli cell-free and whole cell expression systems.
TL;DR: A comparison of in vitro and in vivo expression suggests that despite lower yields and less pure protein preparations, bacterial in vitro protein expression coupled with single-step affinity purification offers a rapid, efficient alternative for the high-throughput screening of clones for protein expression and solubility.
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Building a human kinase gene repository: bioinformatics, molecular cloning, and functional validation.
Jaehong Park,Yanhui Hu,T. V. S. Murthy,Fredrik O. Vannberg,Binghua Shen,Andreas Rolfs,Jessica E. Hutti,Lewis C. Cantley,Joshua LaBaer,Ed Harlow,Leonardo Brizuela +10 more
TL;DR: The mining of public databases to collect the sequence information of all identified human kinase genes and the cloning of the corresponding ORFs is described, which will facilitate the functional assaying of this important gene family in phenotypic screens and their use in biochemical and structural studies.
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A Full-Genomic Sequence-Verified Protein-Coding Gene Collection for Francisella tularensis
T. V. S. Murthy,Andreas Rolfs,Yanhui Hu,Zhenwei Shi,Jacob Raphael,Donna Moreira,Fontina Kelley,Seamus McCarron,Daniel Jepson,Elena Taycher,Dongmei Zuo,Stephanie E. Mohr,Mauricio Fernandez,Leonardo Brizuela,Joshua LaBaer +14 more
TL;DR: An automated pipeline is reported that enabled the construction of the first comprehensive sequence-verified plasmid clone resource for more than 96% of protein coding sequences of the genome of F. tularensis, a highly virulent human pathogen and the causative agent of tularemia.