Hepatic ischemia/reperfusion injury has so far been investigated in various experimental models. A clinical transfer of experimental results is, however, problematic because of anatomical and physiological differences and also the inevitable simplification of experimental work. The choice of model must therefore be adapted to the clinical question to be answered. The simplest procedure for inducing normothermic ischemia is to clamp the hepatoduodenal ligament. Models that do not avert portal congestion are regarded as unsuitable. Our current understanding of the pathogenesis of ischemia/reperfusion injury depends mainly on studies whose authors have investigated either global liver ischemia with a portocaval shunt, spleen transposition and in the isolated perfused system, or partial ischemia. This review is a critical examination of various approaches to the study of normothermic hepatic ischemia in experimental animals.

Experimental models of temporary normothermic liver ischemia.

Marine algae are one of the largest producers of biomass in the marine environment. They produce a wide variety of chemically active metabolites in their surroundings, potentially as an aid to protect themselves against the other settling organisms. These seaweeds are an immense source of bioactive molecules for the exploration of novel drugs. The present investigation deals with the screening of phytochemical composition, fatty acid composition and antioxidant activity of the green edible seaweed Caulerpa (Family: Caulerpaceae) (TSN: 6973 & APHIA ID: 211475). The seaweed was collected from Mandapam Coast, Tamil Nadu. different solvents were used for the preparation of seaweed extract. The Phytochemical constituents of seaweeds, such as carbohydrate, protein, tannins, phytosterols, glycosides, alkaloids, flavonoids, diterpenes, resins and saponins of C. /entillifera were analyzed using standard methods. Fatty acid profile was investigated using Gas chromatography Mass spectrometry (GC -MS). Various fatty acid components are analyzed using GC -MS and nearly 35 components were recorded. In the present investigation, the in -vitro analysis of antioxidant property has been confirmed. From this study, it is evident that Caulerpa lentillifera contains various bioactive compounds and can be recommended as seaweed of phytopharmaceutical importance. The seaweed extracts showed high antioxidant activity which is influenced by its phytochemical and fatty acid contents.

SCREENING OF PHYTOCHEMICALS, FATTY ACID COMPOSITION AND IN-VITRO ANALYSIS OF ANTIOXIDANT PROPERTY OF GREEN EDIBLE SEAWEED CAULERPA LENTILLIFERA (FAMILY: CAULERPACEAE)

Cytoprotective effect of hepatic cell pretreatment with 15-deoxyspergualin to prevent warm ischemic reperfusion injuries in rats.

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Cytoprotective effect of hepatic cell pretreatment with 15-deoxyspergualin to prevent warm ischemic reperfusion injuries in rats.