Sunil Kumar
Imperial College London
53 Papers
239 Citations
Sunil Kumar is an academic researcher from Imperial College London. The author has contributed to research in topics: Fluorescence-lifetime imaging microscopy & Microscopy. The author has an hindex of 18, co-authored 53 publications. Previous affiliations of Sunil Kumar include Francis Crick Institute.
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Papers
Multiplexed FRET to Image Multiple Signaling Events in Live Cells
David M. Grant,Wei Zhang,Ewan J. McGhee,Tom D. Bunney,Clifford Talbot,Sunil Kumar,Ian Munro,Christopher Dunsby,Mark A. A. Neil,Matilda Katan,Paul M. W. French +10 more
TL;DR: The combination of spectral ratiometric imaging of ECFP/Venus and high-speed FLIM-FRET of TagRFP/mPlum can increase the spectral bandwidth available and provide robust imaging of multiple FRET sensors within the same cell and since FLIM does not require equal stoichiometries of donor and acceptor, this approach can be used to report on both unimolecular FRET biosensors and protein-protein interactions with the samecell.
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Screening for protein-protein interactions using Förster resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM)
Anca Margineanu,Jia Jia Chan,Douglas J. Kelly,Sean C. Warren,Delphine Flatters,Sunil Kumar,Matilda Katan,Christopher Dunsby,Paul M. W. French +8 more
TL;DR: The application to identify binding partners of MST1 kinase and estimate interaction strength among the members of the RASSF protein family, which have important roles in apoptosis via the Hippo signalling pathway, is demonstrated.
High plasma membrane lipid order imaged at the immunological synapse periphery in live T cells.
Dylan M. Owen,Stephane Oddos,Sunil Kumar,Daniel M. Davis,Mark A. A. Neil,Paul M. W. French,Michael L. Dustin,Anthony I. Magee,Marek Cebecauer +8 more
TL;DR: It is found that higher membrane order resides at the immunological synapse periphery where proximal signalling through the immunoreceptors and accessory proteins in microclusters has previously been shown to take place.
Genetic and biased agonist-mediated reductions in β-arrestin recruitment prolong cAMP signaling at glucagon family receptors.
Ben Jones,Emma Rose McGlone,Zijian Fang,Phil Pickford,Ivan R. Corrêa,Atsuro Oishi,Ralf Jockers,Asuka Inoue,Sunil Kumar,Frederik Görlitz,Christopher Dunsby,Paul M. W. French,Guy A. Rutter,Guy A. Rutter,Tricia Tan,Alejandra Tomas,Stephen R. Bloom +16 more
TL;DR: This study provides a rationale for the development of GLP-1R, GIPR, and GCGR agonists with reduced β-arrestin recruitment, but further work is needed to maximally exploit this strategy for therapeutic purposes.
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Optically sectioned imaging by oblique plane microscopy
TL;DR: Oblique Plane Microscopy (OPM) is a light sheet microscopy technique that combines oblique illumination with correction optics that tilt the focal plane of the collection system as discussed by the authors.