Sun-Hee Jung
Laboratory of Molecular Biology
12 Papers
370 Citations
Sun-Hee Jung is an academic researcher from Laboratory of Molecular Biology. The author has contributed to research in topics: Immunotoxin & Antigen. The author has an hindex of 11, co-authored 12 publications.
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Papers
Engineering interchain disulfide bonds into conserved framework regions of Fv fragments: improved biochemical characteristics of recombinant immunotoxins containing disulfide-stabilized Fv.
TL;DR: The dsFv immunotoxins were more stable in human serum and more resistant to thermal and chemical denaturation than the single chain (sc) F v immunotoxin and may be more useful than scFv Immunotoxin as therapeutic and diagnostic agents.
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Stabilization of the Fv fragments in recombinant immunotoxins by disulfide bonds engineered into conserved framework regions
TL;DR: It should be feasible to use the positions the authors have identified in the conserved framework region to disulfide-stabilize many different Fv's, and in some cases they may have better binding.
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Patent
Recombinant disulfide-stabilized polypeptide fragments having binding specificity
Ira H. Pastan,Byungkook Lee,Sun-Hee Jung,Ulrich Brinkmann +3 more
- 14 Jun 1994
TL;DR: In this paper, disulfide-stabilized recombinant polypeptide molecules which have the binding ability and specificity for another peptide, such as the variable region of an antibody molecule, are described.
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Disulfide stabilization of antibody Fv: computer predictions and experimental evaluation
TL;DR: This work constructs dsFv immunotoxins in which the Fv moiety is fused to a truncated form of Pseudomonas exotoxin, and demonstrates that position H44-L105 is the only one which gives high production yields of active ds Fvs and all other positions gave either low yields and activity or completely failed to produce active dSFv.
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Rapid humanization of the Fv of monoclonal antibody B3 by using framework exchange of the recombinant immunotoxin B3(Fv)-PE38.
TL;DR: Humanized B3(Fv)-PE38 lost immunogenic epitopes recognized by sera from monkeys that had been immunized with B3 (Fv-PE38), and this basic design resulted in some 20-fold loss of activity.
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