Significant growth of the biodiesel industry has lead to an increased availability of its principal by-product crude glycerol. This study deals with the production of lactic acid, using biodiesel w...

Effect of major and minor nutrients on lactic acid production using biodiesel waste-derived crude glycerol as a carbon source by Lactobacillus casei NCIM 2125

Production of value-added products from biodiesel industry waste-derived semi-purified glycerol

Glycerol generated as the by-product of biodiesel production from vegetable oil or animal fat, has increased the availability of crude glycerol in the market. New strategies need to be developed for the utilization of this abundant carbon source. Using biodiesel waste derived semipurified glycerol as the carbon source, Bacillus amyloliquefaciens, Bacillus megaterium and Bacillus cereus were compared for the production of protease, an industrially important enzyme. Results showed that the medium with 1% semipurified glycerol was an effective carbon source for protease production by these organisms. In addition to glycerol concentration, the parameters such as temperature, pH, incubation period and volume of the inoculum were optimized to increase the protease production. Among the three Bacillus species studied, B. amyloliquefaciens was found to be the best producer of protease. A maximum protease production of 543.95 ± 1.84 U/mL occurred at 48 h with the pH of the medium 9.0, and an inoculum density of 2.6 × 10 cells mL at 45°C by B. amyloliquefaciens.

Production of protease from biodiesel waste derived semipurified glycerol

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Production of protease from biodiesel waste derived semipurified glycerol