A reader for a lateral flow test device includes a tray or drawer, extendable from the reader, which receives the test device. The tray includes a calibration test pattern affixed or printed thereon placed proximate to the test device and in alignment with the axis of the test device. As the tray is closed and the test device is inserted to the reader, the calibration test pattern is first read by an optics unit including a photodiode. The resulting photodiode output provides a calibration curve S that the reader then uses to correct for any non-linear response of the reader's optical or electronic systems, thus insuring that every reader will yield the same readout for a given test cartridge, despite reader-to-reader variations or reader degradation with time. One use of the reader is for detection of SARS-CoV-2 infection.

Lateral flow immunoassay test reader and method of use

The invention discloses novel coronavirus rapid detection test paper and a detection method. The novel coronavirus rapid detection test paper is composed of a sample pad, a colloidal gold pad, a reaction color development area, a water absorption area and a supporting plate; the sample pad and the water absorption pad are arranged on the supporting plate and located on the two opposite sides of the supporting plate respectively, and the sample pad is suction filter paper or a glass fiber membrane and used for bearing an object to be detected; a nitrocellulose membrane is fixed on the supporting plate, and the absorbent pad is absorbent paper; through double binding of a novel coronavirus antibody and a receptor ACE2 protein of a virus, a coronavirus protein antigen is specifically capturedand bound by adopting double enzyme-linked immunosorbent assay; the ACE2 antibody in the test paper reaction color development area is combined with biotin, avidin is connected with horse radish peroxidase, and through a biotin-avidin system, rapid judgment can be achieved through chemical color development, and the virus content can also be accurately detected through a microplate reader.

Novel coronavirus rapid detection test paper and detection method

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Novel coronavirus rapid detection test paper and detection method