Stephen J. Perry
University of Sussex
6 Papers
48 Citations
Stephen J. Perry is an academic researcher from University of Sussex. The author has contributed to research in topics: Lymnaea & FMRFamide. The author has an hindex of 6, co-authored 6 publications.
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Papers
Neuronal expression of an FMRFamide-gated Na+ channel and its modulation by acid pH.
TL;DR: The presence of an FMRFamide-gated sodium current with features expected for a FaNaC: amiloride sensitivity, sodium selectivity, specificity for F MRFamide and Phe-Leu-Arg-Phe-amide (FLRFamide), and no dependency on G-protein coupling is demonstrated.
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Neural Modulation of Gut Motility by Myomodulin Peptides and Acetylcholine in the Snail Lymnaea
Stephen J. Perry,Volko A. Straub,György Kemenes,Niovi Santama,Belinda M. Worster,Julian F. Burke,Paul R. Benjamin +6 more
TL;DR: Coapplication of all five myomodulin peptides gave a greater increase in tonus than that produced by the peptide applied individually, suggesting that corelease of the peptides onto the gut would produce an enhanced response.
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Myomodulin Gene of Lymnaea: Structure, Expression, and Analysis of Neuropeptides
Elaine Kellett,Stephen J. Perry,Niovi Santama,Belinda M. Worster,Paul R. Benjamin,Julian F. Burke +5 more
TL;DR: In situ hybridization analysis indicates that the gene is expressed in specific cells in all ganglia of the CNS of Lymnaea, which will allow physiological analysis of the function of myomodulins at the level of single identified neurons.
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Small cardioactive peptide gene: structure, expression and mass spectrometric analysis reveals a complex pattern of co-transmitters in a snail feeding neuron.
TL;DR: Identical stimulatory activity for the two SCP peptides was demonstrated by their application to the isolated foregut, suggesting that their co‐release from the B2 cells may play an important part in the co‐modulation of gut motility, together with acetylcholine and the myomodulin family of peptides.
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Extender PCR: a method for the isolation of sequences regulating gene expression from genomic DNA.
TL;DR: A new polymerase chain reaction (PCR)-based method for “walking” into previously uncloned regions of genomic DNA that negates the need for synthesis of double-stranded vectorette linkers, blocked oligonucleotide adaptors or genomic DNA library construction and screening is described.
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