Stephen C. Smith
University of California, Berkeley
5 Papers
48 Citations
Stephen C. Smith is an academic researcher from University of California, Berkeley. The author has contributed to research in topics: Caulobacter crescentus & Phosphorylation. The author has an hindex of 4, co-authored 5 publications.
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Papers
Harnessing type I CRISPR–Cas systems for genome engineering in human cells
Peter Cameron,Mary M. Coons,Sanne E. Klompe,Alexandra M. Lied,Stephen C. Smith,Bastien Vidal,Paul Daniel Donohoue,Tomer Rotstein,B. Kohrs,David B. Nyer,Rachel Kennedy,Lynda M. Banh,Carolyn Williams,Mckenzi S. Toh,Matthew J. Irby,Leslie S. Edwards,Chun Han Lin,Arthur L.G. Owen,Tim Künne,John van der Oost,Stan J. J. Brouns,Stan J. J. Brouns,Euan M. Slorach,Chris R. Fuller,Scott Gradia,Steven B. Kanner,Andrew May,Samuel H. Sternberg +27 more
TL;DR: This work demonstrates that highly abundant, previously untapped type I CRISPR–Cas systems can be harnessed for genome engineering applications in eukaryotic cells.
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Mutations that Alter RcdA Surface Residues Decouple Protein Localization and CtrA Proteolysis in Caulobacter crescentus
TL;DR: It is shown that localization of RcdA can be decoupled from its effects on CtrA degradation, and epistasis experiments are used to show that RCDA is still required for regulated Ctr a proteolysis when all SsrA-tagged proteins, abundant substrates of ClpXP, are absent from the cell.
23
A Bioassay for the Detection of Perchlorate in the ppb Range
TL;DR: The efficacy of the developed bioassay was demonstrated by analyzing samples from 2-17000 ppb in deionized lab water, tap water, and contaminated groundwater, and the method of standard additions is used to analyze tap water and groundwater samples.
22
Cell cycle-dependent adaptor complex for ClpXP-mediated proteolysis directly integrates phosphorylation and second messenger signals
Stephen C. Smith,Kamal Kishore Joshi,Justin J. Zik,Katherine Trinh,Aron Kamajaya,Peter Chien,Kathleen R. Ryan +6 more
TL;DR: It is demonstrated that proteins needed for rapid CtrA proteolysis in vivo form a phosphorylation-dependent and cyclic diguanylate (cdG)-dependent adaptor complex that accelerates C trA degradation in vitro by ClpXP and is the first example of a multiprotein, cdG-dependent proteolytic adaptor.