Stephanie E. Westcot
Mayo Clinic
5 Papers
77 Citations
Stephanie E. Westcot is an academic researcher from Mayo Clinic. The author has contributed to research in topics: Zebrafish & Gene. The author has an hindex of 5, co-authored 5 publications. Previous affiliations of Stephanie E. Westcot include University of Minnesota.
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Papers
In vivo protein trapping produces a functional expression codex of the vertebrate proteome
Karl J. Clark,Darius Balciunas,Darius Balciunas,Hans Martin Pogoda,Yonghe Ding,Stephanie E. Westcot,Stephanie E. Westcot,Victoria M. Bedell,Tammy M. Greenwood,Mark D. Urban,Kimberly J. Skuster,Andrew M. Petzold,Andrew M. Petzold,Jun Ni,Aubrey L. Nielsen,Ashok Patowary,Vinod Scaria,Sridhar Sivasubbu,Sridhar Sivasubbu,Xiaolei Xu,Matthias Hammerschmidt,Stephen C. Ekker,Stephen C. Ekker +22 more
TL;DR: A conditional in vivo protein-trap mutagenesis system that reveals spatiotemporal protein expression dynamics and can be used to assess gene function in the vertebrate Danio rerio and reports a collection of 350 zebrafish lines that include diverse molecular loci.
Lessons from morpholino-based screening in zebrafish
TL;DR: The application of MOs in zebrafish (Danio rerio) for in vivo functional characterization of gene activity is described and the importance of off-target effect management and thorough downstream phenotypic validation is emphasized.
177
Nicotine response genetics in the zebrafish
Andrew M. Petzold,Darius Balciunas,Darius Balciunas,Sridhar Sivasubbu,Sridhar Sivasubbu,Karl J. Clark,Victoria M. Bedell,Stephanie E. Westcot,Stephanie E. Westcot,Shelly R. Myers,Gary L. Moulder,Mark J. Thomas,Stephen C. Ekker +12 more
TL;DR: A nicotine behavioral assay in zebrafish is developed and applied in a forward genetic screen using gene-breaking transposon mutagenesis, and it is shown this insertional method generates mutant alleles that are reversible through Cre-mediated recombination, representing a conditional mutation system for the zebra fish.
136
Diversification of stem cell molecular repertoire by alternative splicing
TL;DR: Computational and experimental methods are combined to identify splice variants in embryonic and hematopoietic stem cells on a genome-wide scale and reveal extensive modification of the stem cell molecular repertoire by alternative splicing and provide insights into its overall role as a mechanism of generating genomic diversity.
82
Protein-Trap Insertional Mutagenesis Uncovers New Genes Involved in Zebrafish Skin Development, Including a Neuregulin 2a-Based ErbB Signaling Pathway Required during Median Fin Fold Morphogenesis.
Stephanie E. Westcot,Stephanie E. Westcot,Julia Hatzold,Mark D. Urban,Stefânia K. Richetti,Kimberly J. Skuster,Rhianna M. Harm,Roberto Lopez Cervera,Noriko Umemoto,Melissa S. McNulty,Karl J. Clark,Matthias Hammerschmidt,Stephen C. Ekker,Stephen C. Ekker +13 more
TL;DR: The Zebrafish Integument Project (ZIP), an expression-driven platform for identifying new skin genes and phenotypes in the vertebrate model Danio rerio, identified eleven new, revertible GBT alleles of genes involved in skin development.