Stefano Bruno
University of Parma
98 Papers
722 Citations
Stefano Bruno is an academic researcher from University of Parma. The author has contributed to research in topics: Chemistry & Medicine. The author has an hindex of 20, co-authored 81 publications.
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Papers
Structures of gamma-aminobutyric acid (GABA) aminotransferase, a pyridoxal 5'-phosphate, and [2Fe-2S] cluster-containing enzyme, complexed with gamma-ethynyl-GABA and with the antiepilepsy drug vigabatrin.
Paola Storici,Daniela De Biase,Francesco Bossa,Stefano Bruno,Andrea Mozzarelli,Caroline M. Peneff,Richard B. Silverman,Tilman Schirmer +7 more
TL;DR: In this paper, the crystal structures of pig liver GABA-AT in its native form and in complex with vigabatrin as well as with the close analogue γ-ethynyl-GABA (to 2.3 and 2.8 A, respectively).
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Mutant p53 prevents GAPDH nuclear translocation in pancreatic cancer cells favoring glycolysis and 2-deoxyglucose sensitivity.
Giovanna Butera,Raffaella Pacchiana,Nidula Mullappilly,Marilena Margiotta,Stefano Bruno,Paola Conti,Chiara Riganti,Massimo Donadelli +7 more
TL;DR: It is demonstrated that mutant p53 prevents the nuclear translocation of the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) stabilizing its cytoplasmic localization, thus supporting glycoleysis of cancer cells and inhibiting cell death mechanisms mediated by nuclear GAPDH.
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CO Rebinding Kinetics and Molecular Dynamics Simulations Highlight Dynamic Regulation of Internal Cavities in Human Cytoglobin
Matteo Gabba,Stefania Abbruzzetti,Francesca Spyrakis,Flavio Forti,Stefano Bruno,Andrea Mozzarelli,F. Javier Luque,Cristiano Viappiani,Cristiano Viappiani,Pietro Cozzini,Marco Nardini,Francesca Germani,Martino Bolognesi,Luc Moens,Sylvia Dewilde +14 more
TL;DR: A substantial change in both protein dynamics and inner cavities is observed upon transition from the CO-liganded to the pentacoordinated and bis-histidyl hexacoordination species, which could be exploited as a signalling state consistent with the expected versatility of the molecular activity of this protein.
Experiments on Hemoglobin in Single Crystals and Silica Gels Distinguish among Allosteric Models.
Eric R. Henry,Andrea Mozzarelli,Cristiano Viappiani,Cristiano Viappiani,Stefania Abbruzzetti,Stefania Abbruzzetti,Stefano Bettati,Luca Ronda,Stefano Bruno,William A. Eaton +9 more
TL;DR: It is shown that only the tertiary two-state model can provide a near quantitative explanation of the single-crystal and gel experimental results of hemoglobin trapping experiments.
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ATP binding to human serine racemase is cooperative and modulated by glycine.
Marialaura Marchetti,Stefano Bruno,Barbara Campanini,Alessio Peracchi,Nicole Mai,Andrea Mozzarelli +5 more
TL;DR: Results indicate cross‐talk between allosteric and active sites, leading to the stabilization of two alternative protein conformations with ATP affinities of ~ 10 μM and 1.8 mm, as evaluated within the Monod, Wyman and Changeux model.
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