Spyros Darmanis
Stanford University
79 Papers
114 Citations
Spyros Darmanis is an academic researcher from Stanford University. The author has contributed to research in topics: Biology & Proximity ligation assay. The author has an hindex of 24, co-authored 59 publications. Previous affiliations of Spyros Darmanis include Royal Institute of Technology & Science for Life Laboratory.
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Papers
Single-Cell RNAseq analysis of infiltrating neoplastic cells at the migrating front of human glioblastoma
Spyros Darmanis,Steven A. Sloan,Derek Croote,Marco Mignardi,Sophia B. Chernikova,Peyman Samghababi,Ye Zhang,Norma F. Neff,Mark Kowarsky,Christine Caneda,Gordon Li,Steven L. Chang,Ian D. Connolly,Yingmei Li,Ben A. Barres,Melanie Hayden Gephart,Stephen R. Quake +16 more
TL;DR: It is found that infiltrating GBM cells share a consistent gene signature between patients, suggesting a common mechanism of infiltration, and transcriptionally distinct myeloid cell populations residing in the tumor core and the surrounding peritumoral space are found.
Heterogeneity and targeted therapy-induced adaptations in lung cancer revealed by longitudinal single-cell RNA sequencing
Ashley Maynard,Caroline E. McCoach,Julia K Rotow,Lincoln Harris,Franziska Haderk,Lucas D Kerr,Elizabeth A. Yu,Erin L. Schenk,Weilun Tan,Alexander Zee,Michelle Tan,Philippe Gui,Tasha Lea,Wei Wu,Anatoly Urisman,Kirk D. Jones,Rene Sit,Pallav K. Kolli,Eric J. Seeley,Yaron Gesthalter,Daniel D. Le,Kevin A. Yamauchi,David M Naeger,Nicholas J. Thomas,Anshal Gupta,Mayra Gonzalez,Hien Do,Lisa Tan,Rafael Gomez-Sjoberg,Matthew A. Gubens,Thierry Jahan,Johannes R. Kratz,David M. Jablons,Norma F. Neff,Robert C. Doebele,Jonathan S. Weissman,Collin M. Blakely,Spyros Darmanis,Trever G. Bivona +38 more
TL;DR: How therapy-induced adaptation of the multi-cellular ecosystem of metastatic cancer shapes clinical outcomes is highlighted, with scRNAseq revealing biological features of clinical outcomes in independent cohorts.
Correction: Corrigendum: Solid-phase proximity ligation assays for individual or parallel protein analyses with readout via real-time PCR or sequencing
Rachel Yuan Nong,Di Wu,Junhong Yan,Maria Hammond,Gucci Jijuan Gu,Masood Kamali-Moghaddam,Ulf Landegren,Spyros Darmanis +7 more
TL;DR: In the version of this article initially published, the PCR table at Step 34C(iii) was incorrect and the sliding splint sequence in Supplementary Table 3 was also incorrect.
Ageing compromises mouse thymus function and remodels epithelial cell differentiation
Jeanette Baran-Gale,Morgan,Morgan,Stefano Maio,Stefano Maio,Fatima Dhalla,Fatima Dhalla,Irene Calvo-Asensio,Mary E. Deadman,Mary E. Deadman,Adam E. Handel,Ashley Maynard,Steven Chen,Foad Green,Rene Sit,Norma F. Neff,Spyros Darmanis,Weilun Tan,AP May,John C. Marioni,John C. Marioni,John C. Marioni,Chris P. Ponting,Chris P. Ponting,Georg A. Holländer +24 more
TL;DR: It is shown that mouse ageing leads to less efficient T cell selection, decreased self-antigen representation and increased T cell receptor repertoire diversity, and that progenitor cells are the principal targets of ageing, whereas the function of mature thymic epithelial cells is compromised only modestly.
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PS1 FAD mutants decrease ephrinB2-regulated angiogenic functions, ischemia-induced brain neovascularization and neuronal survival
Yonejung Yoon,Georgios Voloudakis,Nathan Doran,Emily Zhang,Christina Dimovasili,Lei Chen,Zhiping Shao,Spyros Darmanis,Cheuk Y. Tang,Jun Tang,Victoria X. Wang,Patrick R. Hof,Nikolaos K. Robakis,Anastasios Georgakopoulos +13 more
TL;DR: Together, the data show that PS1 FAD mutations impede the EphB4/ephrinB2-mediated angiogenic functions of ECs and impair brain neovascularization, neuronal survival and cognitive recovery following ischemia.
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