Simran Arora
University of Pittsburgh
7 Papers
38 Citations
Simran Arora is an academic researcher from University of Pittsburgh. The author has contributed to research in topics: Chromodomain & Methyllysine. The author has an hindex of 5, co-authored 7 publications. Previous affiliations of Simran Arora include Indian Institute of Science Education and Research, Kolkata & Indian Institute of Science Education and Research, Bhopal.
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Papers
Complementary Steric Engineering at the Protein-Ligand Interface for Analogue-Sensitive TET Oxygenases.
TL;DR: This work provides a general platform for developing analogue-sensitive 2KG-dependent oxygenases to unravel their functions in diverse signaling processes and demonstrates application of the orthogonal mutant-inhibitor pair to probe transcriptional activity of a specific TET member in cells.
Site- and degree-specific C-H oxidation on 5-methylcytosine homologues for probing active DNA demethylation.
Sam Kavoosi,Babu Sudhamalla,Debasis Dey,Kirsten Shriver,Simran Arora,Sushma Sappa,Kabirul Islam +6 more
TL;DR: Activity of TET, AID and TDG enzymes in the DNA demethylation pathway was controlled using stereoelectronically constrained 5-methylcytosine homologues to generate conditionally stable DNA modification.
Structural Ensemble of CD4 Cytoplasmic Tail (402-419) Reveals a Nearly Flat Free-Energy Landscape with Local α-Helical Order in Aqueous Solution.
TL;DR: This work carried out extensive replica exchange molecular dynamics simulations in explicit water with three popular protein force fields Amber ff99SB, Amber ff 99SB*-ILDN, and CHARMM36 to characterize the equilibrium conformational ensemble of CD4-tail and constructed Markov state model (MSM) from large-scale molecular dynamics simulation to study the dynamics of transitions between different metastable states explored by this peptide.
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Engineering a methyllysine reader with photoactive amino acid in mammalian cells.
TL;DR: A chromodomain variant is engineer to carry 4-azido-l-phenylalanine (AzF) via amber suppressor mutagenesis and demonstrated its potential to bind and crosslink methylated proteins in human cells and develops a first-of-its-kind variant bearing two AzF units with enhanced crosslinking potential suitable for profiling the transient methyllysine interactome.
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Allele-Specific Inhibition of Histone Demethylases
TL;DR: This study developed demethylase variants with an expanded active site capable of performing lysine demethylation with wild‐type proficiency, but are sensitive to inhibition by cofactor‐competitive molecules embellished with a complementary steric “bump”.
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