Shusei Obata
Hokkaido University
8 Papers
120 Citations
Shusei Obata is an academic researcher from Hokkaido University. The author has contributed to research in topics: Site-directed mutagenesis & Peptide sequence. The author has an hindex of 5, co-authored 8 publications. Previous affiliations of Shusei Obata include University of Tokyo.
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Papers
A microbial factory for lactate-based polyesters using a lactate-polymerizing enzyme.
Seiichi Taguchi,Miwa Yamada,Ken'ichiro Matsumoto,Kenji Tajima,Yasuharu Satoh,Masanobu Munekata,Katsuhiro Ohno,Katsunori Kohda,Takashi Shimamura,Hiromi Kambe,Shusei Obata +10 more
TL;DR: The engineered PHA synthase gene was introduced into the resultant recombinant strain and confirmed the one-step biosynthesis of the LA-incorporated copolyester, P, with a number-average molecular weight of 1.9 × 105, as revealed by gel permeation chromatography, gas chromatography/MS, and NMR.
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Molecular cloning and nucleotide sequence determination of gene encoding Streptomyces subtilisin inhibitor (SSI).
TL;DR: A gene for Streptomyces subtilisin inhibitor (SSI) from StrePTomyces albogriseolus S-3253 was cloned into E. coli plasmid pBR322 using two oligodeoxyribonucleotides corresponding to Asp68 to Pro77 and Asn99 to Gly107 of the protein, respectively.
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Alteration of the Specificity of the Streptomyces Subtilisin Inhibitor by Gene Engineering
TL;DR: By altering only one amino acid residue at the reactive site of SSI to the substrate specificity of the respective protease, Streptomyces subtilisin inhibitor could successfully change its inhibitory profile.
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High-level expression in Streptomyces lividans 66 of a gene encoding Streptomyces subtilisin inhibitor from Streptomyces albogriseolus S-3253.
TL;DR: A secretory expression system for Streptomyces subtilisin inhibitor (SSI) was established in a heterologous host, StrePTomyces lividans 66, by introducing the 1.8-kbp BglII/SalI fragment containing SSI gene into the Streptomeces multicopy vector, pIJ 702.
27
Patent
Method for production of polylactate using recombinant microorganism
Shusei Obata,Hiromi Kambe,Masakazu Ito,Takashi Shimamura,Katsunori Kohda,Seiichi Taguchi +5 more
- 27 Oct 2009
TL;DR: In this paper, a method for efficiently producing a polylactate via microbial fermentation with the use of a sugar as a starting material is presented. But the method is not suitable for polylactic acid.
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