Shunsuke F. Shimobayashi
Princeton University
20 Papers
65 Citations
Shunsuke F. Shimobayashi is an academic researcher from Princeton University. The author has contributed to research in topics: Lipid bilayer & DNA. The author has an hindex of 8, co-authored 20 publications. Previous affiliations of Shunsuke F. Shimobayashi include Kyoto University & École Normale Supérieure.
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Papers
Nucleated transcriptional condensates amplify gene expression.
Ming-Tzo Wei,Yi-Che Chang,Shunsuke F. Shimobayashi,Yongdae Shin,Yongdae Shin,Amy R. Strom,Clifford P. Brangwynne,Clifford P. Brangwynne +7 more
TL;DR: It is found that TAF15 has a unique charge distribution among the FET family members that enhances its interactions with the C-terminal domain of RNA polymerase II, suggesting that positive feedback between interacting transcriptional components drives localized phase separation to amplify gene expression.
Nucleation landscape of biomolecular condensates.
Shunsuke F. Shimobayashi,Pierre Ronceray,Pierre Ronceray,David W. Sanders,Mikko Haataja,Clifford P. Brangwynne,Clifford P. Brangwynne +6 more
TL;DR: In this paper, the authors quantitatively characterize the nucleation kinetics of endogenous and biomimetic condensates in living cells, and find that key features of condensate nucleation can be quantitatively understood through a CNT-like theoretical framework.
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Suppression of the coffee-ring effect by sugar-assisted depinning of contact line
TL;DR: The findings show that suppressing the coffee-ring effect by adding sugar is a cost-effective, easy and nontoxic strategy for improving the pattern resolution.
Properties of repression condensates in living Ciona embryos
Nicholas Treen,Shunsuke F. Shimobayashi,Jorine M. Eeftens,Clifford P. Brangwynne,Clifford P. Brangwynne,Michael Levine +5 more
TL;DR: The authors developed an expression assay in living Ciona embryos that captures the liquid behavior of individual nucleoli undergoing dynamic fusion events, and used it to visualize puncta of Hes repressors, along with the Groucho/TLE corepressor.
Nucleated transcriptional condensates amplify gene expression
Ming-Tzo Wei,Yi-Che Chang,Shunsuke F. Shimobayashi,Shunsuke F. Shimobayashi,Yongdae Shin,Yongdae Shin,Clifford P. Brangwynne,Clifford P. Brangwynne +7 more
TL;DR: It is shown that amino acid sequence-dependent phase separation of TAF15 is enhanced significantly due to strong nuclear interactions with the C-terminal domain (CTD) of RNA Pol II, which suggests a model in which positive feedback between key transcriptional components drives intermittent dynamics of localized phase separation, to amplify gene expression.
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