The development of alternatives to soil stabilization through mechanical and chemical stabilization has paved the way for the development of biostabilization methods. Since its development, researchers have used different bacteria species for soil treatment. Soil treatment through bioremediation techniques has been used to understand its effect on strength parameters and contaminant remediation. Using a living organism for binding the soil grains to make the soil mass dense and durable is the basic idea of soil biotreatment. Bacteria and enzymes are commonly utilized in biostabilization, which is a common method to encourage ureolysis, leading to calcite precipitation in the soil mass. Microbial-induced calcite precipitation (MICP) and enzyme-induced calcite precipitation (EICP) techniques are emerging trends in soil stabilization. Unlike conventional methods, these techniques are environmentally friendly and sustainable. This review determines the challenges, applicability, advantages, and disadvantages of MICP and EICP in soil treatment and their role in the improvement of the geotechnical and geoenvironmental properties of soil. It further elaborates on their probable mechanism in improving the soil properties in the natural and lab environments. Moreover, it looks into the effectiveness of biostabilization as a remediation of soil contamination. This review intends to present a hands-on adoptable treatment method for in situ implementation depending on specific site conditions.

https://www.mdpi.com/2073-4352/11/4/370/pdf

State-of-the-Art Review of the Applicability and Challenges of Microbial-Induced Calcite Precipitation (MICP) and Enzyme-Induced Calcite Precipitation (EICP) Techniques for Geotechnical and Geoenvironmental Applications

Effects of bacterial inoculation and calcium source on microbial-induced carbonate precipitation for lead remediation

Application of microbial-induced carbonate precipitation (MICP) techniques to remove heavy metal in the natural environment: A critical review.

Study on soil physical structure after the bioremediation of Pb pollution using microbial-induced carbonate precipitation methodology.

Mechanisms, application advances and future perspectives of microbial-induced heavy metal precipitation: A review

Urease producing microorganisms for coal dust suppression isolated from coal: Characterization and comparative study

To promote the application of microbially induced mineralization technology in the field of coal dust suppression, two urease-producing bacteria were co-cultured, with the aim to define the influence of different culture conditions on the growth and urease activity of the bacteria. According to the results, when S. pasteurii and B. cereus CS1 were inoculated in succession at a volume ratio of 1:1 and an interval of 14 h, the mixed bacteria achieved optimal growth and had the highest urease activity; when the initial pH value of culture medium was 9 and the urea and Ca2+ concentrations in the substrate were uniformly 0.1 mol/L, the growth and urease activity of the mixed bacterial culture reached their peaks. SEM-EDS and XRD results indicated that, regardless of the specific urease-producing bacteria used (single urease-producing bacteria or the mixed urease-producing bacteria), their mineralization products were uniformly vaterite-type and calcite-type calcium carbonate; FTIR and thermogravimetric analysis also confirmed their mineralization products as calcium carbonate. By spraying the bacterial inoculants with a corresponding calcium source and urea on pulverized coal, it was found that the bacteria successfully survived and caused pulverized coal to be consolidated. In particular, the mixed bacterial inoculant manifested a stronger consolidation effect, with a wind erosion–induced mass loss of less than 20 g/(m2•h). We provide experimental support for the field of microbial coal dust suppression.

Coal Dust Consolidation Using Calcium Carbonate Precipitation Induced by Treatment with Mixed Cultures of Urease-Producing Bacteria

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Urease producing microorganisms for coal dust suppression isolated from coal: Characterization and comparative study

Coal Dust Consolidation Using Calcium Carbonate Precipitation Induced by Treatment with Mixed Cultures of Urease-Producing Bacteria