Shigeyuki Yokoyama
University of Tokyo
1125 Papers
10.8K Citations
Shigeyuki Yokoyama is an academic researcher from University of Tokyo. The author has contributed to research in topics: Transfer RNA & Thermus thermophilus. The author has an hindex of 107, co-authored 1113 publications. Previous affiliations of Shigeyuki Yokoyama include University of Tsukuba & RIKEN Brain Science Institute.
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Papers
Site-specific fluorescent labeling of RNA by a base-pair expanded transcription system
Rie Kawai,Michiko Kimoto,Tsuneo Mitsui,Shigeyuki Yokoyama,Ichiro Hirao +4 more
- 01 Nov 2004
TL;DR: Ribonucleoside 5'-triphosphates of fluorescently-labeled 2-oxo(1H)pyridines, in which carboxyfluorescein (FAM) or carboxytetramethylrhodamine (TAMRA) was attached at position 5, were chemically synthesized.
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Efforts toward creating unnatural base pairs for an expanded genetic code.
Ichiro Hirao,Tsuneo Mitsui,Tsuyoshi Fujiwara,Michiko Kimoto,Taiko To,Taeko Okuni,Akira Sato,Yoko Harada,Shigeyuki Yokoyama +8 more
- 01 Nov 2001
TL;DR: A series of unnatural base pairs was designed and examined for the expansion of the genetic alphabet and for a better understanding of the mechanism of nucleic acid biosyntheses.
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Importance of Specific Purine-Pyrimidine Amino and Hydroxyl Groups for Efficient Cleavage by a Hammerhead Ribozyme
H Tanaka,Hideo Hosaka,R Takahashi,Y Imamura,Kazuyuki Takai,Shigeyuki Yokoyama,Hiroshi Takaku +6 more
- 01 Jan 1993
TL;DR: This work chemically synthesized and experimentally analyzed chimeric DNA-RNA ribozymes in which the catalytic center remains as RNA.
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Experimental determination of orientations for the 17O electric-field-gradient and chemical shielding tensors in l-alanine
TL;DR: The present results suggest that the intermediate EFG tensor components, VYY, lie in the carboxylate plane and parallel to the C-O bond directions, while the least shielded components, delta11, and the intermediate CS tensor component, delta22, roughly lie inThe molecular plane and the direction of delta22 components are approximately 38 degrees and 25 degrees off theC-O bonds for O1 and O2, respectively.
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Crystallization and preliminary X-ray crystallographic study of leucyl-tRNA synthetase from the archaeon Pyrococcus horikoshii.
TL;DR: The leucyl-tRNA synthetase (LeuRS) from the archaeon Pyrococcus horikoshii was overexpressed in a C-terminally truncated form in Escherichia coli, purified and crystallized by the hanging-drop vapour-diffusion method using ammonium sulfate as a precipitant to solve the structure by the SAD phasing method.
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