6 Papers
70 Citations
Shi Lei is an academic researcher from Bowling Green State University. The author has contributed to research in topics: Azotobacter vinelandii & Gene. The author has an hindex of 4, co-authored 6 publications.
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Papers
Genetic analysis of nif regulatory genes by utilizing the yeast two-hybrid system detected formation of a NifL-NifA complex that is implicated in regulated expression of nif genes.
TL;DR: A genetic approach, the use of a yeast two-hybrid protein-protein interaction assay system, is taken, to investigate NifL interaction with NifA, and analyses showed that the kinase-like domain of NIFL directly interacts with the catalytic domain of nifA.
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Nif Phenotype of Azotobacter vinelandii UW97 CHARACTERIZATION AND MUTATIONAL ANALYSIS
TL;DR: A systematic analysis of an A. vinelandii UW97 mutant is reported and it seems that the Nif phenotype is caused by a general structural disturbance of the Fe protein due to the presence of the bulky phenylalanine at position 44.
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Identification of a second site compensatory mutation in the Fe-protein that allows diazotrophic growth of Azotobacter vinelandii UW97.
TL;DR: Computational analysis showed that the Fe‐protein mutant that retained serine 44 but contained the substitution of lysine at position 170 by glutamic acid was also non‐functional; however, theFe‐protein in A. vinelandii BG9 that contained both substitutions could support diazotrophic growth on the strain.
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Activation of vanadium nitrogenase expression in Azotobacter vinelandii DJ54 revertant in the presence of molybdenum.
TL;DR: Analysis of promoter activity of different nif systems revealed that the vnf promoter is activated in A. vinelandii BG54, and it is concluded that this mutant strain utilizes vf nitrogenase proteins to support its diazotrophic growth.
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Molecular investigations on the dilemma of nitrogen‐fixing characteristics of Azotomonas
TL;DR: The investigations on the nitrogen fixation characteristics of Azotomonas fluorescens strain ATCC13544 show that it can grow in both liquid medium and on solid agar plates made with modified BURK nitrogen‐free medium supplemented with ammonium acetate as a nitrogen source.
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