Scott Cayley
University of Wisconsin-Madison
9 Papers
25 Citations
Scott Cayley is an academic researcher from University of Wisconsin-Madison. The author has contributed to research in topics: Osmolyte & Macromolecular crowding. The author has an hindex of 9, co-authored 9 publications.
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Papers
K(+)-ribosome interactions determine the large enhancements of 39K NMR transverse relaxation rates in the cytoplasm of Escherichia coli K-12.
TL;DR: K(+)-ribosome interactions are the dominant source of the NMR properties of K+ in E. coli, and 39K and 23Na exhibit the large magnitudes and the large difference between the slow and the fast relaxation rates observed in viable cells.
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Biophysical compensation mechanisms buffering E. coli protein–nucleic acid interactions against changing environments
TL;DR: Biophysical effects resulting from changes in the amount of cytoplasmic water and in the concentrations of other cytopalasmic solutes appear to compensate for the effects of changes in cytopLasmic K+ concentration and thereby maintain protein-nucleic acid equilibria and kinetics in the range required for in vivo function.
Characterization of the cytoplasm of Escherichia coli K-12 as a function of external osmolarity. Implications for protein-DNA interactions in vivo.
TL;DR: The water-accessible volumes, the amounts of all significant osmolytes, and the protein concentration in the cytoplasm of aerobically grown Escherichia coli K-12 have been determined as a function of the osmolarity of the minimal growth medium as discussed by the authors.
Large changes in cytoplasmic biopolymer concentration with osmolality indicate that macromolecular crowding may regulate protein-DNA interactions and growth rate in osmotically stressed Escherichia coli K-12.
Scott Cayley,M. Thomas Record +1 more
TL;DR: It is proposed that crowding increases with increasing growth osmolality, which in turn buffers the binding of proteins to nucleic acids against changes in cytoplasmic K+ concentration and is a determinant of growth rate of osmotically stressed cells.