Scott A. Gabel
Research Triangle Park
6 Papers
135 Citations
Scott A. Gabel is an academic researcher from Research Triangle Park. The author has contributed to research in topics: Binding site & Importin. The author has an hindex of 6, co-authored 6 publications.
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Papers
Nuclear magnetic resonance measurement of cytosolic free calcium levels in human red blood cells.
TL;DR: Red blood cells were loaded with 1,2-bis(2-amino-5-fluorophenoxy)ethane-N, N,N,N',N'-tetraacetic acid by incubation with 50 microM of the acetoxymethyl ester, and cytosolic free Ca2+ was monitored with 19F-nuclear magnetic resonance (NMR).
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Interligand Overhauser effects in type II dihydrofolate reductase.
Dawei Li,L. A. Levy,Scott A. Gabel,Mark S. Lebetkin,Eugene F. DeRose,Mark J. Wall,Elizabeth E. Howell,Robert E. London +7 more
TL;DR: The results support a structure in which the NADP(+) and folate/DMDDF molecules extend in opposite directions parallel to the long axis of the pore, with the nicotinamide and pterin ring systems approximately stacked at the center.
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Nuclear Localization of the DNA Repair Scaffold XRCC1: Uncovering the Functional Role of a Bipartite NLS.
Thomas W. Kirby,Natalie R. Gassman,Cassandra E. Smith,Lars C. Pedersen,Scott A. Gabel,Mack Sobhany,Samuel H. Wilson,Robert E. London +7 more
TL;DR: Crystallography and binding studies confirm the bipartite nature of the XRCC1 NLS interaction with Importin α (Impα) in which the major and minor binding motifs are separated by >20 residues, and resolve previous inconsistent determinations.
NMR-sensitive fluorinated and fluorescent intracellular calcium ion indicators with high dissociation constants.
TL;DR: A new series of high-dissociation constant Ca2+ indicators has been developed to reduce perturbations due to buffering of transients, to carry out measurements in cells and organelles with high basal Ca2- concentrations, and to measure cytosolic Ca 2+ levels in the presence of perturbation that may significantly increase these levels.
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Measurement of cytosolic free calcium in perfused rat heart using TF-BAPTA.
TL;DR: TF-BAPTA is a useful new indicator for measuring elevations in Ca2+i or compartmentation of Ca2-i, and it is shown that the rise in Ca 2+i is not uniform during prolonged global ischemia (60 min); several different Ca2 +i values are present.
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