Affiliations: Department of Safety Research on Blood and Biological Products, National Institute of Infectious Diseases, Tokyo, 208-0011, Japan. National Hospital Organization, Murayama Medical Center, Tokyo 208-0011, Japan. Department of Virology 1, National Institute of Infectious Diseases; Tokyo 162-8640, Japan. Research Center for Drug and Vaccine Development, National Institute of Infectious Diseases; Tokyo, 162-8640, Japan. Department of Veterinary Science, National Institute of Infectious Diseases; Tokyo, 162-8640, Japan. Department of Pathology, National Institute of Infectious Diseases; Tokyo, 162-8640, Japan. These authors contributed equally Lead Contact

Safety and immunogenicity of Pfizer/BioNTech SARS-CoV-2 mRNA third booster vaccine against SARS-CoV-2 Omicron variant in Japanese healthcare workers Authors:

Highlights A novel assay system for evaluation of Fc g R-effector function in cynomolgus macaque PBMCs Several different cell types bound to HA-expressing cells in the Fc g R-dependent manner IgGs elicited by ﬂu vaccination induced Fc g R-dependent classical monocytes binding This assay system could facilitate the development of a universal inﬂuenza vaccine SUMMARY Several cross-protective antibodies that recognize a broad range of inﬂuenza A virus (IAV) strains are known to have functions in virus elimination such as Fc g receptor (Fc g R)-effector function and neutralizing activity against the head region. Although few studies have used primary cells as effector cells, the Fc g R-effector function was evaluated after isolating each cell subset. Herein, we established an original assay system to evaluate puriﬁed FI6 IgG-mediated binding to hemagglutinin (HA)-expressing cells by ﬂow cytometry using peripheral blood mononuclear cells from cynomolgus macaques. In addition, we evaluated the Fc g R-effector function of IAV vaccine-induced anti-HA antibodies in cynomolgus macaques after administering the split vaccine. We found several cell types, mainly classical monocytes, bound to HA-expressing target cells in an Fc g R-dependent manner, that were dominant in the binding of the cell population. Thus, this assay system could facilitate the development of a universal inﬂuenza vaccine. administration. The results showed that the antibodies induced by the split vaccine mediate FcR-dependent target-effector binding, and the main subset of the bound cell is classical monocytes. This assay system will be helpful for the development of a universal inﬂuenza vaccine because it will allow us to examine in detail the Fc g R-effector function of individual cells induced by antibodies from passive immunization or vaccine administration in NHPs.

Assessment of Fc g receptor-dependent binding of inﬂuenza hemagglutinin vaccine-induced antibodies in a non-human primate model

Handle everyday research tasks with reliable, citation-backed results

Your personal Research Agent to handle research tasks with citation-backed results

Popular Tasks used by Researchers

How can I help with your research?

Meet SciSpace

Get more enhanced response by uploading the PDFs you want me to reference.

No relevant PDFs in your library

SciSpace is the AI research assistant for academics. Run systematic literature reviews on 280M+ papers, and write papers with cited sources. Trusted by 1M+ students, PhDs & researchers.

SciSpace | AI for Research

Analyze PDFs

Code & Manuscripts

Funding & Grants

Literature & Patents

Medical & Clinical Data

Systematic Review

Visualize & Present

Web & Data

Build a Google Scholar-like website for your research.

Build a website

Create charts and images for your research

Create a Chart

Write a paper for submission to a journal

Draft a manuscript

Patent Search

Design eye-catching scientific posters in minutes.

Scientific Poster Generation

Systematic Literature Review

One task is running at the moment. Your messages will be shown right after.

Drag and drop or click here to browse

Loved by <highlight>1 million+</highlight> researchers

Extract a list of specific topics and their sources from unstructured text

Topics

Compare and analyze relevant papers that matches with your search

Papers

Get insights from PDFs and bookmarked papers from your library

My library

Recent searches

Try searching for:

Catch AI-generated content in scholarly and non-scholarly content

{ai} Detector

Ai Writer

Get PDF Summaries, highlighted text explanations 

Chat with PDF

Effortlessly create in-text citations and bibliographies in APA and 2,500 other formats

Citation generator

Get explanations, summaries, and answers on academic papers

Ease up your research workflow with {scispace}'s cohort of exciting AI tools

Elevate your academic writing skills and convey your ideas the way you want

Paraphraser

Explore our range of reading and writing tools

Your file is being prepared and should be ready in a few minutes. If it's a large file, it might take a bit longer. You can close this window, and we'll email you the file when it's done.

You have reached a maximum limit of <strong>{limit}</strong> columns in the table. Remove at least <strong>1</strong> column to add or create another one.

Saya

Author Tools

Chat about Author

Papers

Safety and immunogenicity of Pfizer/BioNTech SARS-CoV-2 mRNA third booster vaccine against SARS-CoV-2 Omicron variant in Japanese healthcare workers Authors:

Assessment of Fc g receptor-dependent binding of inﬂuenza hemagglutinin vaccine-induced antibodies in a non-human primate model