Savan K. Patel
University of Pennsylvania
5 Papers
11 Citations
Savan K. Patel is an academic researcher from University of Pennsylvania. The author has contributed to research in topics: Chemistry & Medicine. The author has co-authored 2 publications.
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Papers
Orthogonal Design of Experiments for Optimization of Lipid Nanoparticles for mRNA Engineering of CAR T Cells.
Margaret M. Billingsley,Alex G. Hamilton,David Mai,Savan K. Patel,Kelsey L. Swingle,Neil C. Sheppard,Carl H. June,Michael J. Mitchell +7 more
TL;DR: In this article, sequential libraries of ionizable lipid nanoparticle (LNP) formulations with varied excipient compositions were screened in comparison to a standard formulation for improved mRNA delivery to T cells with low cytotoxicity, revealing B10 as the top formulation with a 3-fold increase in mRNA delivery.
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Hydroxycholesterol substitution in Ionizable lipid nanoparticles for mRNA delivery to T cells.
Savan K. Patel,Margaret M. Billingsley,C. M. Frazee,Xuexiang Han,K. Swingle,Jingya Qin,Mohamad-Gabriel Alameh,Karin Wang,Drew Weissman,Michael J. Mitchell +9 more
TL;DR: In this article , a library of ionizable lipid nanoparticles (LNPs) incorporating hydroxycholesterols was used to evaluate their impact on mRNA delivery to T cells by leveraging endosomal trafficking mechanisms.
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In Vivo mRNA CAR T Cell Engineering via Targeted Ionizable Lipid Nanoparticles with Extrahepatic Tropism.
Margaret M. Billingsley,Ningqiang Gong,Alvin J. Mukalel,Ajay S. Thatte,Rakan El-Mayta,Savan K. Patel,Ann E. Metzloff,K. Lynn Swingle,Xuexiang Han,Lulu Xue,Alex G. Hamilton,Hannah Safford,Mohamad-Gabriel Alameh,Tyler E Papp,Hamideh Parhiz,Drew Weissman,Michael J. Mitchell +16 more
TL;DR: This work conjugates antibodies to LNPs with extrahepatic tropism, evaluates pan-T cell markers, and develops Ab-LNPs capable of generating functional CAR T cells in vivo.
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Bile acid-containing lipid nanoparticles enhance extrahepatic mRNA delivery
Savan K. Patel,Margaret M. Billingsley,Alvin J. Mukalel,Ajay S. Thatte,Alex G. Hamilton,Ningqiang Gong,Rakan El-Mayta,Hannah Safford,Maria Merolle,Michael J. Mitchell +9 more
TL;DR: Bile acid-containing LNPs (BA-LNPs) were able to reduce delivery to liver cells in vitro and improve delivery in a variety of other cell types, including T cells, B cells, and epithelial cells, suggesting that this cholic acid replacement strategy may be generalizable.
Amniotic fluid stabilized lipid nanoparticles for in utero intra-amniotic mRNA delivery.
Kelsey L. Swingle,William H. Peranteau,Jingbin Wang,Margaret M. Billingsley,Sourav Bose,Brandon White,Rohan Palanki,Apeksha Dave,Savan K. Patel,Ningqiang Gong,Alex G. Hamilton,Mohamad-Gabriel Alameh,Drew Weissman,William H. Peranteau,Michael J. Mitchell +14 more
TL;DR: In this paper, a library of ionizable lipid nanoparticles (LNPs) was used to evaluate how LNP structure affects ex-natal stability in amniotic fluid, and whether a lead candidate identified from these stability measurements enables intra-amniotic mRNA delivery in utero.
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