Sarah Kutscher
Technische Universität München
7 Papers
54 Citations
Sarah Kutscher is an academic researcher from Technische Universität München. The author has contributed to research in topics: Immune system & CD8. The author has an hindex of 5, co-authored 7 publications.
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Papers
Overnight Resting of PBMC Changes Functional Signatures of Antigen Specific T- Cell Responses: Impact for Immune Monitoring within Clinical Trials
Sarah Kutscher,Claudia J. Dembek,Simone Deckert,C Russo,Nina Körber,Johannes R. Bogner,Fabian Geisler,Andreas Umgelter,Michael Neuenhahn,Julia Albrecht,Antonio Cosma,Ulrike Protzer,Tanja Bauer +12 more
TL;DR: Overnight resting of PBMC prior to ex vivo analysis of antiviral T-cell responses represents an efficient method to increase sensitivity of ICS-based methods and has a prominent impact on the functional phenotype of T cells.
Design of therapeutic vaccines: hepatitis B as an example.
TL;DR: Options to develop a therapeutic vaccine for chronic viral infections using HBV as a promising example are described and induction of a multi‐specific and multifunctional T‐cell response against key viral antigens is a paradigm of therapeutic vaccination.
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The intracellular detection of MIP-1beta enhances the capacity to detect IFN-gamma mediated HIV-1-specific CD8 T-cell responses in a flow cytometric setting providing a sensitive alternative to the ELISPOT
Sarah Kutscher,Claudia J. Dembek,Simone Allgayer,Silvia Heltai,Birgit Stadlbauer,Priscilla Biswas,Silvia Nozza,Giuseppe Tambussi,Johannes R. Bogner,Hans Jürgen Stellbrink,Frank D. Goebel,Paolo Lusso,Marco Tinelli,Guido Poli,Volker Erfle,Heike Pohla,Mauro S. Malnati,Antonio Cosma +17 more
TL;DR: The IFN-γ+ MIP-1β+ data evaluation system provides a clear advantage for the detection of low magnitude HIV-1-specific responses and is important to guide the choice for suitable highly sensitive immune assays and to build reagent panels able to accurately characterize the phenotype and function of responding T-cells.
MVA- nef induces HIV-1-specific polyfunctional and proliferative T-cell responses revealed by the combination of short- and long-term immune assays
Sarah Kutscher,Simone Allgayer,Claudia J. Dembek,Johannes R. Bogner,Ulrike Protzer,Frank-D. Goebel,Volker Erfle,Antonio Cosma +7 more
TL;DR: The results highlight the importance of combining sophisticated immunomonitoring tools to unravel concealed effects of immunological interventions and support the use of the poxvirus-derived MVA vector to stimulate highly functional HIV-1-specific T-cell responses, however, the clinical benefit of these functional T cells remains to be determined.
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Nef-specific CD45RA+ CD8+ T cells secreting MIP-1beta but not IFN-gamma are associated with nonprogressive HIV-1 infection.
Claudia J. Dembek,Sarah Kutscher,Silvia Heltai,Simone Allgayer,Priscilla Biswas,Silvia Ghezzi,Elisa Vicenzi,Dieter Hoffmann,Peter Reitmeir,Giuseppe Tambussi,Johannes R. Bogner,Paolo Lusso,Hans Jürgen Stellbrink,Elena Santagostino,Thomas Vollbrecht,Frank D. Goebel,Ulrike Protzer,Rika Draenert,Marco Tinelli,Guido Poli,Volker Erfle,Mauro S. Malnati,Antonio Cosma +22 more
TL;DR: The novel antigen-specific CD45RA+ IFN-gammaneg MIP-1beta+ CD8+ T cell population represents a new candidate marker of long-term natural control of HIV-1 disease progression and a relevant functional T-cell subset in the evaluation of the immune responses induced by candidate HIV- 1 vaccines.
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