Sam Kavoosi
University of Pittsburgh
5 Papers
37 Citations
Sam Kavoosi is an academic researcher from University of Pittsburgh. The author has contributed to research in topics: Oligonucleotide & DNA demethylation. The author has an hindex of 2, co-authored 4 publications.
Chat about Author
Papers
Complementary Steric Engineering at the Protein-Ligand Interface for Analogue-Sensitive TET Oxygenases.
TL;DR: This work provides a general platform for developing analogue-sensitive 2KG-dependent oxygenases to unravel their functions in diverse signaling processes and demonstrates application of the orthogonal mutant-inhibitor pair to probe transcriptional activity of a specific TET member in cells.
Engineering bromodomains with a photoactive amino acid by engaging ‘Privileged’ tRNA synthetases
Shana Wagner,Babu Sudhamalla,Philip Z. Mannes,Sushma Sappa,Sam Kavoosi,Debasis Dey,Sinan Wang,Kabirul Islam +7 more
TL;DR: 4-(trifluoromethyldiazirinyl)-phenylalanine is resurrected by introducing several key features including easy synthetic access, site-specific incorporation by 'privileged' synthetases and superior crosslinking efficiency, to develop photo-crosslinkable bromodomains suitable for 'interactome' profiling.
10
Site- and degree-specific C-H oxidation on 5-methylcytosine homologues for probing active DNA demethylation.
Sam Kavoosi,Babu Sudhamalla,Debasis Dey,Kirsten Shriver,Simran Arora,Sushma Sappa,Kabirul Islam +6 more
TL;DR: Activity of TET, AID and TDG enzymes in the DNA demethylation pathway was controlled using stereoelectronically constrained 5-methylcytosine homologues to generate conditionally stable DNA modification.
5-Dihydroxyboryluridine enhances cytosolic penetration of antisense oligonucleotides.
TL;DR: In this paper , the authors harness the reversible binding between boronic acids and cell surface glycans to aid in the cellular delivery of synthetic oligonucleotides and demonstrate that these boronate-containing nucleic acids have enhanced cytosolic penetration and splice-correcting activity compared to non-boronate analogs.
4
Synthesis of 5-Dihydroxyboryluridine Phosphoramidite and Its Site-Specific Incorporation into Oligonucleotides for Probing Thymine DNA Glycosylase.
TL;DR: 5boU was introduced into short oligonucleotides in a site-specific manner, demonstrating compatibility of the boronic acid moiety with standard solid-phase DNA synthesis chemistry.
4