Sam Cooper
Imperial College London
14 Papers
Sam Cooper is an academic researcher from Imperial College London. The author has contributed to research in topics: Inpainting & DNA damage. The author has an hindex of 11, co-authored 14 publications. Previous affiliations of Sam Cooper include Institute of Cancer Research.
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Papers
Data-analysis strategies for image-based cell profiling
Juan C. Caicedo,Sam Cooper,Florian Heigwer,Scott Warchal,Peng Qiu,Csaba Molnar,Aliaksei Vasilevich,Joseph Barry,Harmanjit Singh Bansal,Oren Kraus,Mathias Wawer,Lassi Paavolainen,Markus D. Herrmann,Mohammad Hossein Rohban,Jane Hung,Jane Hung,Holger Hennig,John Concannon,Ian Smith,Paul A. Clemons,Shantanu Singh,Paul Rees,Paul Rees,Peter Horvath,Peter Horvath,Roger G. Linington,Anne E. Carpenter +26 more
TL;DR: The steps required to create high-quality image-based (i.e., morphological) profiles from a collection of microscopy images are introduced and techniques that have proven useful in each stage of the data analysis process are recommended on the basis of the experience of 20 laboratories worldwide that are refining their image- based cell-profiling methodologies.
DNA damage during S-phase mediates the proliferation-quiescence decision in the subsequent G1 via p21 expression
Alexis R. Barr,Sam Cooper,Sam Cooper,Frank S. Heldt,Francesca Butera,Henriette Stoy,Jörg Mansfeld,Bela Novak,Chris Bakal +8 more
TL;DR: It is shown that naturally occurring DNA damage incurred over S-phase causes p53-dependent accumulation of p21 during mother G2- and daughter G1-phases, and mathematical modelling reveals that a bistable switch, created by CRL4Cdt2, promotes irreversible S- Phase entry by keeping p21 levels low, preventing premature S- phase exit upon DNA damage.
369
Nanoneedle-Mediated Stimulation of Cell Mechanotransduction Machinery
Catherine S. Hansel,Spencer W. Crowder,Sam Cooper,Sahana Gopal,Maria João Pardelha Da Cruz,Leonardo de Oliveira Martins,Debora Keller,Stephen Rothery,Michele Becce,Anthony E. G. Cass,Chris Bakal,Ciro Chiappini,Molly M. Stevens +12 more
TL;DR: It is shown that mesoporous silicon nanoneedle arrays interact simultaneously with the cell membrane, cytoskeleton, and nucleus of primary human cells, generating distinct responses at each of these cellular compartments.
127
Learning unsupervised feature representations for single cell microscopy images with paired cell inpainting
TL;DR: This work introduces a convolutional neural network (CNN) to automatically design features for fluorescence microscopy, and uses a selfsupervised method to learn feature representations of single cells in microscopy images without labelled training data.
RNAi screens for Rho GTPase regulators of cell shape and YAP/TAZ localisation in triple negative breast cancer
Patricia Pascual-Vargas,Sam Cooper,Julia E. Sero,Vicky Bousgouni,Mar Arias-Garcia,Chris Bakal +5 more
TL;DR: An RNAi screen is performed to isolate RhoGEFs and RhoGAPs that contribute to the morphogenesis of the highly metastatic TNBC cell line LM2, and its less-metastatic parental cell line MDA-MB-231 and describes the morphological heterogeneity of each gene-depleted population.