Ryan Prestil
University of Wisconsin-Madison
9 Papers
33 Citations
Ryan Prestil is an academic researcher from University of Wisconsin-Madison. The author has contributed to research in topics: Induced pluripotent stem cell & Biology. The author has an hindex of 5, co-authored 7 publications. Previous affiliations of Ryan Prestil include National Institutes of Health & University of Cambridge.
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Papers
Transcription Factor-Mediated Differentiation of Human iPSCs into Neurons.
Michael S. Fernandopulle,Ryan Prestil,Christopher Grunseich,Chao Wang,Li Gan,Michael E. Ward +5 more
TL;DR: A set of protocols to assist investigators in the culture and genetic engineering of iPSC lines to enable transcription factor–mediated differentiation ofiPSCs into i3Neurons or i3LMNs are described and presented, and neuronal culture conditions for various experimental applications are presented.
Autophagy induction as a therapeutic strategy for neurodegenerative diseases
TL;DR: Evidence in cells and in vivo demonstrates promising results in many disease models, in which autophagy upregulation is able to reduce the levels of toxic proteins, ameliorate signs of disease, and delay disease progression.
203
High-Content Analysis of CRISPR-Cas9 Gene-Edited Human Embryonic Stem Cells
Jared Carlson-Stevermer,Madelyn Goedland,Benjamin Steyer,Arezoo Movaghar,Meng Lou,Lucille Kohlenberg,Ryan Prestil,Krishanu Saha +7 more
TL;DR: This preclinical platform adds important capabilities to observe editing and selection in situ within complex structures generated by human cells, ultimately enabling optical and other molecular perturbations in the editing workflow that could refine the specificity and versatility of gene editing.
26
Tracking and Predicting Human Somatic Cell Reprogramming Using Nuclear Characteristics.
Kaivalya Molugu,Ty Harkness,Jared Carlson-Stevermer,Ryan Prestil,Nicole J. Piscopo,Stephanie K. Seymour,Gavin T. Knight,Randolph S. Ashton,Krishanu Saha +8 more
TL;DR: This approach to track reprogramming in situ using micropatterned substrates could aid in biomanufacturing of therapeutically relevant iPSCs and be used to elucidate multiscale cellular changes (cell-cell interactions as well as subcellular changes) that accompany human cell fate transitions.
9
Multi-modal Proteomic Characterization of Lysosomal Function and Proteostasis in Progranulin-Deficient Neurons
Saadia Hasan,Michael S. Fernandopulle,Stewart W. Humble,Ashley M Frankenfield,Haorong Li,Ryan Prestil,Kory R. Johnson,Brent J. Ryan,Richard Wade-Martins,Michael E. Ward,Ling Hao +10 more
TL;DR: In this paper , the impact of progranulin deficiency on lysosomal biology in neurons was investigated using multi-modal techniques and tools to study the highly dynamic Lysosome biology.