Ryan J. Lake
University of Illinois at Urbana–Champaign
14 Papers
5 Citations
Ryan J. Lake is an academic researcher from University of Illinois at Urbana–Champaign. The author has contributed to research in topics: Chemistry & Deoxyribozyme. The author has an hindex of 6, co-authored 11 publications.
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Papers
DNAzymes as Activity-Based Sensors for Metal Ions: Recent Applications, Demonstrated Advantages, Current Challenges, and Future Directions.
TL;DR: The DNAzyme-based sensors allow for in vitro selection to expand the method to almost any metal ion under a variety of conditions, negative selection to improve the selectivity against competing targets, and reselection of DNAzymes and combination of active and inactive variants to fine-tune the dynamic range of detection.
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Imaging Endogenous Metal Ions in Living Cells Using a DNAzyme-Catalytic Hairpin Assembly Probe.
Zhenkun Wu,Zhenkun Wu,Huanhuan Fan,Huanhuan Fan,Nitya Sai Reddy Satyavolu,Wenjing Wang,Wenjing Wang,Ryan J. Lake,Jian-Hui Jiang,Yi Lu +9 more
TL;DR: The design of a catalytic hairpin assembly (CHA) reaction to amplify the signal from photocaged Na+ -specific DNAzyme to detect endogenous Na+ inside cells is reported to contribute to deeper understanding of the role of metal ions in biological systems.
Enzyme-Mediated Endogenous and Bioorthogonal Control of a DNAzyme Fluorescent Sensor for Imaging Metal Ions in Living Cells.
TL;DR: The endogenous and bioorthogonal activation of a DNAzyme fluorescent sensor containing an 18-base pair recognition site of a homing endonuclease is reported, demonstrating the use of I- Sce I to activate the 10-23 DNAzyme for imaging of Mg 2+ in HeLa cells with biorthogonal and endogenous control.
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A photo-regulated aptamer sensor for spatiotemporally controlled monitoring of ATP in the mitochondria of living cells
TL;DR: This work represents the first successful delivery of a DNA aptamer sensor to mitochondria, providing a new platform for targeted delivery to subcellular organelles for monitoring energy producing processes, as well as mitochondrial dysfunction-related diseases in different cells.
Metal-Dependent DNAzymes for the Quantitative Detection of Metal Ions in Living Cells: Recent Progress, Current Challenges, and Latest Results on FRET Ratiometric Sensors.
TL;DR: This Forum Article summarizes recent progress made in developing these DNAzyme sensors to probe metal ions in living cells and in vivo, including several challenges that were able to overcome for this application, such as DNAzyme delivery, spatiotemporal control, and signal amplification.
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