Ryan D. Kitko
Kenyon College
6 Papers
48 Citations
Ryan D. Kitko is an academic researcher from Kenyon College. The author has contributed to research in topics: Bacillus subtilis & Escherichia coli. The author has an hindex of 6, co-authored 6 publications.
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Papers
Acid and Base Stress and Transcriptomic Responses in Bacillus subtilis
Jessica C. Wilks,Ryan D. Kitko,Sarah H. Cleeton,Grace E. Lee,Chinagozi S. Ugwu,Brian D. Jones,Sandra Splinter BonDurant,Joan L. Slonczewski +7 more
TL;DR: Great genetic adaptation was seen at pH 9 than at pH 6, which may explain the lag time required for growth shift to high pH, and the SigH, SigL, and SigW regulons were upregulated at high pH.
Cytoplasmic pH Response to Acid Stress in Individual Cells of Escherichia coli and Bacillus subtilis Observed by Fluorescence Ratio Imaging Microscopy
Keith A. Martinez,Ryan D. Kitko,J. Patrick Mershon,Haley E. Adcox,Kotiba A. Malek,Melanie B. Berkmen,Joan L. Slonczewski +6 more
TL;DR: Overall, the cytoplasmic pHs of individual bacteria show important variation in the acid stress response, including novel responses in biofilms.
Cytoplasmic acidification and the benzoate transcriptome in Bacillus subtilis.
Ryan D. Kitko,Rebecca Cleeton,Erin I. Armentrout,Grace E. Lee,Ken Noguchi,Melanie B. Berkmen,Brian D. Jones,Joan L. Slonczewski +7 more
TL;DR: B. subtilis maintains partial pH homeostasis during growth, and withstands high concentrations of permeant acid stress, higher than for gram-negative neutralophile E. coli.
Osmolytes Contribute to pH Homeostasis of Escherichia coli
TL;DR: Diverse osmolytes including NaCl, KCl, proline, or sucrose contribute to cytoplasmic pH homeostasis in E. coli, and increase the recovery from rapid acid shift.
A Requirement of TolC and MDR Efflux Pumps for Acid Adaptation and GadAB Induction in Escherichia coli
Kari N. W. Deininger,Akina Horikawa,Ryan D. Kitko,Ryoko Tatsumi,Judah L. Rosner,Masaaki Wachi,Joan L. Slonczewski +6 more
TL;DR: TolC and proton-driven MDR efflux pump components EmrB and MdtB contribute to E. coli survival in extreme acid and TolC is required for maximal growth rates below pH 6.5, an important consideration for enteric organisms passing through the acidic stomach.