Rumi Ohtake
Tokyo University of Agriculture
6 Papers
6 Citations
Rumi Ohtake is an academic researcher from Tokyo University of Agriculture. The author has contributed to research in topics: Bran & Microsatellite. The author has an hindex of 3, co-authored 6 publications.
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Papers
Effective DNA fragmentation technique for simple sequence repeat detection with a microsatellite-enriched library and high-throughput sequencing.
TL;DR: Comparison of the two DNA fragmentation techniques showed that restriction enzyme digestion was superior to sonication for identification of heterozygous genotypes, whereas sonication was superior for detection of various SSR flanking regions with both species-specific and common characteristics.
6
Draft Genome Sequence of Paenibacillus amylolyticus Heshi-A3, Isolated from Fermented Rice Bran in a Japanese Fermented Seafood Dish.
Sayuri Akuzawa,Junko Nagaoka,Motoki Kanekatsu,Eri Kubota,Rumi Ohtake,Tomonori Suzuki,Yu Kanesaki +6 more
TL;DR: The draft genome sequence of Paenibacillus amylolyticus strain Heshi-A3 is reported, isolated in Fukui prefecture, Japan, from fermented rice bran in Heshiko, a traditional dish that is produced by aging salted mackerel with fresh riceBran at an ambient temperature for around 7 months to over one year.
5
Draft Genome Sequence of Bacillus licheniformis Heshi-B2, Isolated from Fermented Rice Bran in a Japanese Fermented Seafood Dish.
Yu Kanesaki,Yu Kanesaki,Eri Kubota,Rumi Ohtake,Yukari Higashi,Junko Nagaoka,Tomonori Suzuki,Sayuri Akuzawa +7 more
TL;DR: The draft genome sequence of B. licheniformis Heshi-B2 is reported, originating from a Heshiko sample from Fukui Prefecture, Japan, which was isolated from fermented rice bran in Heshikos, a food produced by aging salted mackerel with fresh riceBran.
2
A naturally occurring insertion in BrEMS1 confers genic male sterility in turnip (Brassica rapa ssp. rapa)
TL;DR: The results indicate that the male sterility phenotype of ms77B is due to a null mutation of BrEMS1, which is essential for anther development, which has a long insertion in the coding sequence, and RNA sequencing revealed that the gene is not transcribed beyond the insertion site.
Microsatellite Capture Sequencing
Keisuke Tanaka,Rumi Ohtake,Saki Yoshida,Takashi Shinohara +3 more
- 20 Jun 2018
TL;DR: The protocol used in this study demonstrates the possibility of using low-input DNA (≥1 ng), and while the use of restriction enzymes was more suitable for identifying the heterozygous genotype than sonication was, sonication facilitated the detection of various SSR flanking regions with both species-specific and common characteristics more than restriction enzyme digestion did.