Ruby Lieshout
Erasmus University Rotterdam
16 Papers
10 Citations
Ruby Lieshout is an academic researcher from Erasmus University Rotterdam. The author has contributed to research in topics: Biology & Medicine. The author has an hindex of 6, co-authored 8 publications.
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Papers
Human primary liver cancer–derived organoid cultures for disease modeling and drug screening
Laura Broutier,Gianmarco Mastrogiovanni,Monique M A Verstegen,Hayley E. Francies,Lena Morrill Gavarró,Charles R. Bradshaw,George E. Allen,Robert Arnes-Benito,Olga Sidorova,Marcia P. Gaspersz,Nikitas Georgakopoulos,Bon-Kyoung Koo,Sabine Dietmann,Susan E. Davies,Raaj K. Praseedom,Ruby Lieshout,Jan N. M. IJzermans,Stephen J. Wigmore,Kourosh Saeb-Parsy,Mathew J. Garnett,Luc J. W. van der Laan,Meritxell Huch +21 more
TL;DR: The wide-ranging biomedical utilities of PLC-derived organoid models in furthering the understanding of liver cancer biology and in developing personalized-medicine approaches for the disease are demonstrated.
Cancer-Associated Fibroblasts Provide a Stromal Niche for Liver Cancer Organoids That Confers Trophic Effects and Therapy Resistance
Jiaye Liu,Pengfei Li,Ling Wang,Meng Li,Zhouhong Ge,Lisanne Noordam,Ruby Lieshout,Monique M A Verstegen,Buyun Ma,Junhong Su,Qin Yang,Ruyi Zhang,Guoying Zhou,Lucia Campos Carrascosa,Dave Sprengers,Jan N. M. IJzermans,Ron Smits,Jaap Kwekkeboom,Luc J. W. van der Laan,Maikel P. Peppelenbosch,Qiuwei Pan,Wanlu Cao +21 more
TL;DR: 3D organotypic co-cultures of primary liver tumor–derived organoids with cancer-associated fibroblasts are established and robust effects of CAFs are shown in liver cancer nurturing and treatment resistance.
139
Large-Scale Production of LGR5-Positive Bipotential Human Liver Stem Cells
Kerstin Schneeberger,Natalia Sánchez-Romero,Shicheng Ye,Frank G. van Steenbeek,Loes A Oosterhoff,Iris Pla Palacin,Chen Chen,Chen Chen,Monique E van Wolferen,Gilles S van Tienderen,Ruby Lieshout,Haaike Colemonts-Vroninks,Imre F. Schene,Ruurdtje Hoekstra,Monique M A Verstegen,Luc J. W. van der Laan,Louis C. Penning,Sabine A. Fuchs,Hans Clevers,Hans Clevers,Joery De Kock,Pedro M. Baptista,Bart Spee +22 more
TL;DR: Human organoids derived from leucine‐rich repeat‐containing G protein‐coupled receptor 5 (LGR5)–positive adult stem cells represent an exciting new cell source for liver regeneration; however, culturing large numbers of organoids with current protocols is tedious and the level of hepatic differentiation is limited.
122
Mitochondrial Fusion Via OPA1 and MFN1 Supports Liver Tumor Cell Metabolism and Growth
Meng Li,Ling Wang,Yijin Wang,Shaoshi Zhang,Guoying Zhou,Ruby Lieshout,Buyun Ma,Jiaye Liu,Changbo Qu,Monique M A Verstegen,Dave Sprengers,Jaap Kwekkeboom,Luc J. W. van der Laan,Wanlu Cao,Maikel P. Peppelenbosch,Qiuwei Pan +15 more
TL;DR: Investigation of the mitochondrial morphodynamics and its functional consequences in human liver cancer finds increased mitochondrial fusion in liver cancer alters metabolism and fuels tumor cell growth.
88
Modelling immune cytotoxicity for cholangiocarcinoma with tumour-derived organoids and effector T cells
Guoying Zhou,Ruby Lieshout,Gilles S van Tienderen,Valeska de Ruiter,Martin E. van Royen,Patrick P.C. Boor,Luc Magré,Jyaysi Desai,Kübra Köten,Yik Y. Kan,Zhouhong Ge,Lucia Campos Carrascosa,Cecilia A.W. Geuijen,Dave Sprengers,Luc J. W. van der Laan,Monique M A Verstegen,Jaap Kwekkeboom +16 more
TL;DR: In this paper , the authors developed a co-culture method with patient-derived cholangiocarcinoma (CCA) organoids and immune cells, which could represent anti-cancer immunity in vitro.