Robert W. Johnson
6 Papers
23 Citations
Robert W. Johnson is an academic researcher. The author has contributed to research in topics: Mass spectrometry & Phosphorylation. The author has an hindex of 5, co-authored 6 publications.
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Papers
Structural Characterization of a Soluble Amyloid β-Peptide Oligomer
Liping Yu,Rohinton Edalji,John E. Harlan,Thomas F. Holzman,Ana Pereda Lopez,Boris Labkovsky,Heinz Hillen,Stefan Barghorn,Ulrich Ebert,Paul L. Richardson,Laura J. Miesbauer,Larry R. Solomon,Diane Bartley,Karl A. Walter,Robert W. Johnson,Philip J. Hajduk,Edward T. Olejniczak +16 more
TL;DR: Using NMR, the NMR data indicate that soluble oligomers of Abeta preglobulomer and globulomer have a mixed parallel and antiparallel beta-sheet structure that is different from fibrils which contain only parallel beta-sheets.
367
ALARM NMR: a rapid and robust experimental method to detect reactive false positives in biochemical screens.
Jeffrey R. Huth,Renaldo Mendoza,Edward T. Olejniczak,Robert W. Johnson,Darlene A Cothron,Yaya Liu,Claude G. Lerner,Jun Chen,Philip J. Hajduk +8 more
TL;DR: A rapid and reliable NMR-based method for identifying reactive false positives including those that oxidize or alkylate a protein target and a new filtering tool has been developed on the basis of the ALARM NMR data that can augment current in silico programs for identifying nuisance compounds and improving the process of hit triage.
227
Characterization of protein kinase A phosphorylation: multi-technique approach to phosphate mapping.
Jianwei Shen,Richard A. Smith,Vincent S. Stoll,Rohinton Edalji,Clarissa G. Jakob,Karl A. Walter,Emily Gramling,Sally Dorwin,Diane Bartley,Angelo Gunasekera,Jianguo Yang,Thomas F. Holzman,Robert W. Johnson +12 more
TL;DR: Data from this study suggested that PKA was completely phosphorylated at S10, T197, and S338 and partially phosphorylations at S139, illustrating that critical information can be lost unless multiple MS techniques are used for identification and validation of phosphorylation.
9
Protein fragmentation via liquid chromatography-quadrupole time-of-flight mass spectrometry: the use of limited sequence information in structural characterization
Robert W. Johnson,Tanveer F. Ahmed,Laura J. Miesbauer,Rohinton Edalji,Richard S. Smith,John E. Harlan,Sarah A. Dorwin,Karl A. Walter,T. F. Holzman +8 more
TL;DR: An alternative approach to top-down sequencing using in-source fragmentation on quadrupole time-of-flight (Q-Tof) instrumentation coupled with reversed-phase liquid chromatography (LC) is described, illustrating that intact protein mass and limited sequence information can be obtained simultaneously on an LC timescale.
9
Domain structure analysis of elongation factor‐3 from saccharomyces cerevisiae by limited proteolysis and differential scanning calorimetry
Uri S. Ladror,David A. Egan,Seth W. Snyder,John O. Capobianco,Robert C. Goldman,Sarah A. Dorwin,Robert W. Johnson,Rohinton Edalji,Aparna V. Sarthy,Tom Mcgonigal,Karl A. Walter,Thomas F. Holzman +11 more
TL;DR: Results suggest a tight structure of the N‐terminus, which may give rise to the 58 °C transition, and a looseructure of the C‐ terminus, giving rise toThe 39 °C Transition.