Robert N. Bowles
Icahn School of Medicine at Mount Sinai
6 Papers
27 Citations
Robert N. Bowles is an academic researcher from Icahn School of Medicine at Mount Sinai. The author has contributed to research in topics: Nucleofection & Herpes simplex virus. The author has an hindex of 5, co-authored 6 publications.
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Papers
Loss of Matrix Metalloproteinase-2 Amplifies Murine Toxin-Induced Liver Fibrosis by Upregulating Collagen I Expression
Brian Radbill,Ritu Gupta,Maria Celeste M. Ramirez,Analisa DiFeo,John A. Martignetti,Carlos E. Alvarez,Scott L. Friedman,Goutham Narla,Raluca Vrabie,Robert N. Bowles,Yedidya Saiman,Meena B. Bansal +11 more
TL;DR: It is suggested that increased MMP-2 during the progression of liver fibrosis may be an important mechanism for inhibiting type I collagen synthesis by activated HSCs, thereby providing a protective rather than pathologic role.
100
Validation of efficient high-throughput plasmid and siRNA transfection of human monocyte-derived dendritic cells without cell maturation.
TL;DR: This protocol optimized and validated an effective high-throughput transfection protocol, allowing us to transiently express DNA in naïve primary DCs, as well as investigate the effect of gene silencing by RNA interference, and examined the impact of small interfering RNA-mediated knockdown of RIG-I, a key viral recognition receptor, on the induction of the interferon response in DCs infected with Newcastle disease virus.
16
Reconsideration of viral protein immunoblotting for differentiation of human herpes simplex viruses.
TL;DR: A triple-phase immune-typing procedure is reconsidered and developed that compares differences in electrophoretic mobilities of viral ICP4, ICP27, and VP22 proteins between HSV-1 andHSV-2 strains and is anticipated that this methodology will be useful in distinguishing viruses obtained in clinical cultures.
6
A truncation mutation of the neurovirulence ICP22 protein produced by a recombinant HSV-1 generated by bacterial artificial chromosome technology targets infected cell nuclei.
Robert N. Bowles,John A. Blaho +1 more
TL;DR: HSV-1(BACX) will serve as a useful tool to decipher the unusual biological properties and functions of the ICP22 protein, and showed a reduced replication capacity in rabbit skin cells, consistent with previous studies using I CP22-null viruses.
6
Nucleotidylation to oncoapoptosis
John A. Blaho,Martine Aubert,Kathy Bae,Brooke Baker,Jenny Balderama,Renee Baranin,Monica Bhanot,Amanda Blouin,Robert N. Bowles,Isabella Chi,F. Natalie W. Chirimuuta,Christopher R. Cotter,Elisabeth Gennis,Laura Gillim,Margot L. Goodkin,Kristin Ingvarsdottir,Andrea E. Ireland,Leah Kang,Surinder Kaul,Anna Kotsakis,Rachel M. Kraft,Renzo C. Lambardozzi,Maria R. Lopez,Fatima Manzoor,Kerryn A. Mortimer,Elise R. Morton,Marie L. Nguyen,Jennifer M. O'toole,Jason Paragas,Kristen C. Pena,Lisa E. Pomeranz,Deleen Sabbah,Christine M Sanfilippo,Louis R. Serico,Elisabeth F.M. Schlegel,Chintal Shah,Cecylia Stabrawa,Dennis Vega,Sandra Winstersteller,Jamie C. Yedowitz,C. Susan Zong +40 more
- 01 Mar 2012