Raymond A. Deems
University of California, San Diego
40 Papers
1.1K Citations
Raymond A. Deems is an academic researcher from University of California, San Diego. The author has contributed to research in topics: Phospholipase A2 & Phospholipase. The author has an hindex of 28, co-authored 40 publications. Previous affiliations of Raymond A. Deems include University of California.
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Papers
A Mouse Macrophage Lipidome
Edward A. Dennis,Raymond A. Deems,Richard Harkewicz,Oswald Quehenberger,H. Alex Brown,Stephen B. Milne,David S. Myers,Christopher K. Glass,Gary Hardiman,Donna Reichart,Alfred H. Merrill,M. Cameron Sullards,Elaine Wang,Robert C. Murphy,Christian R. H. Raetz,Teresa A. Garrett,Ziqiang Guan,Andrea C. Ryan,David W. Russell,Jeffrey G. McDonald,Bonne M. Thompson,Walter A. Shaw,Manish Sud,Yihua Zhao,Shakti Gupta,Mano Ram Maurya,Eoin Fahy,Shankar Subramaniam +27 more
TL;DR: The lipidomic response of the murine macrophage RAW cell line to Kdo2-lipid A, the active component of an inflammatory lipopolysaccharide functioning as a selective TLR4 agonist and compactin, a statin inhibitor of cholesterol biosynthesis is reported.
303
Kinetic analysis of phospholipase A2 activity toward mixed micelles and its implications for the study of lipolytic enzymes.
TL;DR: This kinetic treatment has made possible the quantitative separation of the association of a lipolytic enzyme with the lipid-water interface (expressed as KsA) and the binding to the substrate in the interface (reflected in the KmB term).
268
Chemical modification of the histidine residue in phospholipase A2 (Naja naja naja). A case of half-site reactivity.
TL;DR: The results of these chemical modification experiments can be interpreted in terms of a model in which the active species of enzyme interacting with mixed micelles is a dimer (or possibly higher order aggregate).
213
Detection and quantitation of eicosanoids via high performance liquid chromatography-electrospray ionization-mass spectrometry.
Raymond A. Deems,Matthew W. Buczynski,Rebecca C. Bowers-Gentry,Richard Harkewicz,Edward A. Dennis +4 more
TL;DR: A LC-MS/MS procedure for isolating, identifying, and quantitating a broad spectrum of eicosanoids in a single biological sample is developed and a library that includes relative chromatographic retention times and tandem mass spectrometry data for the most common eicOSanoids is established.
Bicelles in structure-function studies of membrane-associated proteins.
TL;DR: This work employed a 31P NMR enzymatic assay system to examine the kinetic behavior of cobra venom phospholipase A(2) toward a variety of bicellar substrates, and found that this PLA( 2) showed no significant preference for long-chain or short-chainospholipids when they were presented as mixtures in bicelle lipids.
116