Background and Aim: Antimicrobials are extensively used in poultry production for growth promotion as well as for the treatment and control of diseases, including avian pathogenic Escherichia coli (APEC). Poor selection, overuse, and misuse of antimicrobial agents may promote the emergence and dissemination of antimicrobial resistance (AMR) in APEC. This study aimed to assess antimicrobial susceptibility patterns and detect antibiotic resistance genes (ARGs) in APEC isolated from clinical cases of colibacillosis in commercial broiler, layer, and breeder chickens. Materials and Methods: A total of 487 APEC were isolated from 539 across 300 poultry farms in various regions of Nepal. Antimicrobial susceptibility patterns was determined using the Kirby-Bauer disk diffusion and broth microdilution methods. The index of AMR, such as multiple antibiotic resistance (MAR) index, resistance score (R-score), and multidrug resistance (MDR) profile, were determined. Polymerase chain reaction was employed to detect multiple ARGs and correlations between phenotypic and genotypic resistance were analyzed. Results: The prevalence of APEC was 91% (487/539). All of these isolates were found resistant to at least one antimicrobial agent, and 41.7% of the isolates were resistant against 8–9 different antimicrobials. The antibiogram of APEC isolates overall showed the highest resistance against ampicillin (99.4%), whereas the highest intermediate resistance was observed in enrofloxacin (92%). The MAR index and R-score showed significant differences between broiler and layers, as well as between broiler breeder and layers. The number of isolates that were resistant to at least one agent in three or more antimicrobial categories tested was 446 (91.6%) and were classified as MDR-positive isolates. The ARGs were identified in 439 (90.1%) APEC isolates, including the most detected mobilized colistin resistance (mcr1) which was detected in the highest (52.6%) isolates. Overall, resistance gene of beta-lactam (blaTEM), mcr1, resistance gene of sulphonamide (sul1) and resistance gene of tetracycline (tetB) (in broiler), were detected in significantly higher than other tested genes (p < 0.001). When examining the pair-wise correlations, a significant phenotype-phenotype correlation (p < 0.001) was observed between levofloxacin and ciprofloxacin, chloramphenicol and tetracycline with doxycycline. Similarly, a significant phenotype-genotype correlation (p < 0.001) was observed between chloramphenicol and the tetB, and colistin with blaTEM and resistance gene of quinolone (qnrA). Conclusion: In this study, the current state of APEC AMR in commercial chickens is revealed for the first time in Nepal. We deciphered the complex nature of AMR in APEC populations. This information of molecular surveillance is useful to combat AMR in APEC and to contribute to manage APEC associated diseases and develop policies and guidelines to enhance the commercial chicken production.

Antimicrobial resistance of avian pathogenic Escherichia coli isolated from broiler, layer, and breeder chickens

Understanding and Management of Fruit Drop in Litchi Cultivation

Food grains infestation by insect pests in stores is a severe challenge in food production around the globe, especially in wet and humid regions. In Bhutan, mung bean is commonly grown for consumption as a superior source of protein. However, severe cowpea weevil infestation is observed while in storage condition. Controlling with synthetic pesticides is associated with health risks due to toxic residues which intervene in safe and healthy protection methods. Protecting the grains in-store through organic approach is imperative for consumption and seed purposes. Some botanical plant extracts are known for their protective properties which need location-specific studies based on availability and suitability. Therefore, this study was conducted to evaluate the efficacy of eight treatments (Acorus calamus rhizome powder, mustard oil, garlic cloves, turmeric rhizome powder, wood ash, Vitex negundo leaf powder, super grain bag including untreated control) against cowpea weevil (Callosobruchus maculatus) in mungbean under storage condition. The experiment was laid in a randomized complete block design with three replications. The result revealed that the lowest mean number of grains perforated and percent grain perforated were recorded in grains treated with Acorus calamus rhizome powder (0.46 & 0.20 %) followed by mustard oil (1.05 & 0.47 %) and grains stored in super grain bag (5.74 & 2.49%), which were significantly lower (P<0.001) as compared to other treatments. The lowest number of adult cowpea weevils was also recorded in grains treated with Acorus calamus and mustard oil followed by grains stored in super grain bag. Germination percentage was found highest in wood ash followed by mustard oil, Acorus calamus and garlic-treated grains while the lowest was in super grain bag. Therefore, Acorus calamus rhizome powder and mustard oil were found to be effective in managing cowpea weevil without affecting seed germination and vigour.

/pdf/efficacy-of-plant-derivatives-in-protecting-mungbean-grains-21o86l1k.pdf

Efficacy of Plant Derivatives in Protecting Mungbean Grains against Cowpea Weevil (Callosobruchus maculatus) under Storage Conditions in Southern Bhutan

Based on a field survey from 2017, twenty-three species are reported from Bhutan for the first time: Acroceratitis ceratitina, A. hardyi, Anomoia approximata, Bactrocera connecta, B. latifrons, B. nigrifacia, B. syzygii, Campiglossa sororcula, Cecidochares connexa, Dacus jacobi, Gastrozona fasciventris, Hoplandromyia antelopa, Lenitovena ultima, Ptilona confinis, Rioxoptilona dunlopi, R. formosana, R. vaga, Spathulina acroleuca, Themara yunnana, Trypeta indica, Zeugodacus apiciflavus, Z. diversus, and Z. fereuncinatus. Four species of the genera Cornutrypeta, Hemilea, Morinowotome, and Vidalia are also recorded for the first time, but the precise determination to species needs additional study and material. As the result, 71 species is listed from Bhutan by far. Their taxonomic position and key characters are discussed. Illustrations for most of the newly recorded species are given.

The Fruit Flies (Diptera, Tephritidae) in Bhutan: New Faunistic Records and Compendium of Fauna

Abstract. Tashi T, Tobgay T, Wangmo T, Kinley R, Gyeltshen S. 2023. Cropping pattern and intensity in the lower belt of Sarpang District, Bhutan. Asian J Agric 7: 1-6. Information on cropping and farming land use is vital for increasing crop production and identifying production gaps, including planning and investment. Increasing cropping intensity through adopting multiple approaches increases crop production rather than pressure on cropland expansion in many countries. In the case of Bhutan, there is a shortage of information on cropping and its intensity in Bhutan. This study presents the status of cropping patterns and cropping intensity at the household level in the Sarpang District using a multistage random sampling technique. Different forms of multiple cropping practices were recorded in the district but were mostly practiced on a small scale and were intermittent. The rice-based cropping pattern was popular in wetlands, while the maize or vegetable-based cropping pattern predominated in dryland. With an average cropping intensity of 112%, the district had a cropping intensity of 97% and 126% for dryland and wetland, respectively. The landholding size revealed a significant inverse relationship with cropping intensity among farmer characteristics. The district's most serious farming problems were a lack of irrigation water and wildlife crop predation. The study recommends a similar study at the national level and developing appropriate intensification of agricultural land use strategy to minimize pressure on cropland expansion in the future. Similarly, agricultural planning and investment merit assessment of crop diversity, crop production resources, cropping intensity gap, and crop mapping.

Cropping pattern and intensity in the lower belt of Sarpang District, Bhutan

In Bhutan, litchi production is constrained by insect pest damage. Among insect pests, the litchi fruit and shoot borer (Conopomorpha sinensis Bradley) is an emerging pest of economic importance in the subtropical regions of Bhutan. Up until now, there is documented research on its biology and management in Bhutan. Therefore, this study was undertaken to study the efficacy of two different management methods against litchi fruit borer infestation in three litchi varieties (Bhur litchi 1, Bhur litchi 2 & Hong houy). The management methods consisted of three treatments; T1-Azadirachtin followed by Imidacloprid, Azadirachtin and Cypermethrin; T2-Azadirachtin followed by Imidacloprid, Azadirachtin and Azadirachtin; T3-Untreated control. The result showed that the fruit infestation at harvest was significantly lower in management method T1 (34.74%) as compared to T2 (78.07%) and untreated control (89.98%). In terms of the variety, the lowest fruit infestation at harvest was observed for Hong houy (56.02%) as compared to Bhur Litchi 1 (73.28%) and Bhur Litchi 2 (73.45%). There were no significant differences in fruit drop percent and percent infestation of dropped fruits between the treatments and varieties tested. Heavy fruit drop was observed in the third week, due to unknown reasons, which then decreased over the fruit development stages. Fruit borer infestation seems to increase rapidly after the fifth and eighth weeks indicating that most of the fruit borer damage occurs at the fruit maturity stage after fruit colour break. Therefore, the last application of cypermethrin 10% EC at the fruit colour break stage was found necessary to reduce fruit infestation during this vulnerable pest infestation period besides scheduled application of Azadirachtin and Imidacloprid at the early fruit development period.

Management of Litchi Fruit and Shoot Borer (Conomorpha sinensis) in Three Litchi Varieties in Samtenling, Bhutan

Handle everyday research tasks with reliable, citation-backed results

Your personal Research Agent to handle research tasks with citation-backed results

Popular Tasks used by Researchers

How can I help with your research?

Meet SciSpace

Get more enhanced response by uploading the PDFs you want me to reference.

No relevant PDFs in your library

SciSpace is the AI research assistant for academics. Run systematic literature reviews on 280M+ papers, and write papers with cited sources. Trusted by 1M+ students, PhDs & researchers.

SciSpace | AI for Research

Analyze PDFs

Code & Manuscripts

Funding & Grants

Literature & Patents

Medical & Clinical Data

Systematic Review

Visualize & Present

Web & Data

Build a Google Scholar-like website for your research.

Build a website

Create charts and images for your research

Create a Chart

Write a paper for submission to a journal

Draft a manuscript

Patent Search

Design eye-catching scientific posters in minutes.

Scientific Poster Generation

Systematic Literature Review

One task is running at the moment. Your messages will be shown right after.

Drag and drop or click here to browse

Loved by <highlight>1 million+</highlight> researchers

Extract a list of specific topics and their sources from unstructured text

Topics

Compare and analyze relevant papers that matches with your search

Papers

Get insights from PDFs and bookmarked papers from your library

My library

Recent searches

Try searching for:

Catch AI-generated content in scholarly and non-scholarly content

{ai} Detector

Ai Writer

Get PDF Summaries, highlighted text explanations 

Chat with PDF

Effortlessly create in-text citations and bibliographies in APA and 2,500 other formats

Citation generator

Get explanations, summaries, and answers on academic papers

Ease up your research workflow with {scispace}'s cohort of exciting AI tools

Elevate your academic writing skills and convey your ideas the way you want

Paraphraser

Explore our range of reading and writing tools

Your file is being prepared and should be ready in a few minutes. If it's a large file, it might take a bit longer. You can close this window, and we'll email you the file when it's done.

You have reached a maximum limit of <strong>{limit}</strong> columns in the table. Remove at least <strong>1</strong> column to add or create another one.

Ratu Kinley

Author Tools

Chat about Author

Papers

Efficacy of Plant Derivatives in Protecting Mungbean Grains against Cowpea Weevil (Callosobruchus maculatus) under Storage Conditions in Southern Bhutan

The Fruit Flies (Diptera, Tephritidae) in Bhutan: New Faunistic Records and Compendium of Fauna

Cropping pattern and intensity in the lower belt of Sarpang District, Bhutan

Management of Litchi Fruit and Shoot Borer (Conomorpha sinensis) in Three Litchi Varieties in Samtenling, Bhutan