Rashmi Tandon
6 Papers
23 Citations
Rashmi Tandon is an academic researcher. The author has contributed to research in topics: Nanopore sequencing & Trinucleotide repeat expansion. The author has an hindex of 4, co-authored 6 publications.
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Papers
Analysis of short tandem repeat expansions and their methylation state with nanopore sequencing
Pay Giesselmann,Björn Brändl,Etienne Raimondeau,Rebecca Bowen,Christian Rohrandt,Rashmi Tandon,Helene Kretzmer,Günter Assum,Christina Galonska,Reiner Siebert,Ole Ammerpohl,Andrew John Heron,Susanne A. Schneider,Julia Ladewig,Philipp Koch,Bernhard Schuldt,James E. Graham,Alexander Meissner,Alexander Meissner,Franz-Josef Müller +19 more
TL;DR: This work demonstrates the precise quantification of repeat numbers in conjunction with the determination of CpG methylation states in the repeat expansion and in adjacent regions at the single-molecule level without amplification.
Generation of two human isogenic iPSC lines from fetal dermal fibroblasts.
Rashmi Tandon,Björn Brändl,Natalya Baryshnikova,Alexandro Landshammer,Laura Steenpaß,Oliver Keminer,Ole Pless,Franz-Josef Müller +7 more
TL;DR: Two isogenic hiPSC lines were generated by reprogramming fetal dermal fibroblasts with episomal vectors, and can be applied as reference cell lines for creating isogenic clones bearing disease mutations on a well-characterized genomic background.
15
Generation of an iPSC line of a patient with Angelman syndrome due to an imprinting defect.
Anika Neureiter,Björn Brändl,Michaela Hiber,Rashmi Tandon,Franz-Josef Müller,Laura Steenpass +5 more
TL;DR: The generated iPSC line was derived from skin fibroblasts of a patient with AS, who, due to an imprinting defect, lacked DNA methylation at the chromosome 15 imprinting center, which controls maternal-specific expression of UBE3A.
8
Repeat expansion and methylation state analysis with nanopore sequencing
Pay Gießelmann,Björn Brändl,Etienne Raimondeau,Rebecca Bowen,Christian Rohrandt,Rashmi Tandon,Helene Kretzmer,Günter Assum,Christina Galonska,Reiner Siebert,Ole Ammerpohl,Andrew John Heron,Susanne A. Schneider,Julia Ladewig,Philipp Koch,Bernhard Schuldt,James E. Graham,Alexander Meissner,Franz-Josef Mueller +18 more
TL;DR: A Cas12a-based enrichment strategy for nanopore sequencing that, combined with a new algorithm for raw signal analysis, enables this work to efficiently target, sequence and precisely quantify repeat numbers as well as their DNA methylation status.
7
Analysis of short tandem repeat expansions and their methylation state with nanopore sequencing
Pay Gießelmann,Björn Brändl,Etienne Raimondeau,Rebecca Bowen,Christian Rohrandt,Rashmi Tandon,Helene Kretzmer,Günter Assum,Christina Galonska,Reiner Siebert,Ole Ammerpohl,Andrew John Heron,Susanne A. Schneider,Julia Ladewig,Philipp Koch,Bernhard Schuldt,James E. Graham,Alexander Meissner,Franz-Josef Müller +18 more
- 01 Dec 2019