Ralph McBride
University of Wyoming
6 Papers
16 Citations
Ralph McBride is an academic researcher from University of Wyoming. The author has contributed to research in topics: Self-healing hydrogels & Cell encapsulation. The author has an hindex of 5, co-authored 6 publications.
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Papers
Microfluidic techniques for high throughput single cell analysis
TL;DR: The microfabrication of microfluidic control systems and the development of increasingly sensitive molecular amplification tools have enabled the miniaturization of single cells analytical platforms, enabling a class of single cell analytical platforms within great potential for data driven biomedicine, genomics and transcriptomics.
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A microfluidic-based cell encapsulation platform to achieve high long-term cell viability in photopolymerized PEGNB hydrogel microspheres
TL;DR: A microfluidic-based droplet fabrication platform that generates consistently monodisperse cell-laden water-in-oil emulsions and long-term cell viability is described and several factors that influence post-encapsulation cell viability were identified.
Composite Hydrogels With Controlled Degradation in 3D Printed Scaffolds
TL;DR: This droplet-microencapsulation technique coupled with composite degradable hydrogel and 3D printing could offer an alternative route for controlled cell delivery with improved cell viability and facile tunability.
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Comparative cytocompatibility of multiple candidate cell types to photoencapsulation in PEGNB/PEGDA macroscale or microscale hydrogels.
TL;DR: PEGNB provides excellent cellular tolerance and supports long-term cell survival by mitigating the deleterious effects of acrylate photopolymerization, which are exacerbated at diminishing volumes, and is an excellent candidate for hydrogel miniaturization.
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Light-inducible activation of cell cycle progression in Xenopus egg extracts under microfluidic confinement.
Jitender S. Bisht,Paige J. LeValley,Paige J. LeValley,Paige J. LeValley,Benjamin Noren,Benjamin Noren,Ralph McBride,Prathamesh M. Kharkar,April M. Kloxin,Jesse C. Gatlin,Jesse C. Gatlin,John Oakey,John Oakey +12 more
TL;DR: Photodegradable polyethylene glycol (PEG) hydrogels are introduced as a vehicle to passively and selectively manipulate extract composition through the release of proteins encapsulated within the hydrogel matrix to present a versatile and customizable route to regulating the spatial and temporal dynamics of cellular events in microfluidically confined cell-free extracts.
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