Sulfate ester formation and hydrolysis: a potentially important yet often ignored aspect of the sulfur cycle of aerobic soils.

1. An enzyme that catalyses the transfer of sulphate from adenosine 3'-phosphate 5'[(35)S]-sulphatophosphate to l-tyrosine methyl ester and tyramine was purified approx. 70-fold from female rat livers. 2. The partially purified preparation is still contaminated with adenosine 3'-phosphate 5'-sulphatophosphate-phenol sulphotransferase (EC 2.8.2.1), but a partial separation of the two enzymes can be achieved by chromatography on columns of Sephadex G-200 and DEAE-Sephadex. 3. The enzyme responsible for the sulphation of l-tyrosine methyl ester and tyramine is activated by dithiothreitol, 2-mercaptoethanol and GSH, the degree of activation being more marked with preparations previously stored at 0 or -10 degrees C. In contrast, the enzymic sulphation of p-nitrophenol is inhibited by all three thiols. Again, there is a quantitative difference in the degree of inhibition of the two enzymes by o-iodosobenzoate, p-chloromercuribenzoate, N-ethylmaleimide and iodoacetate. 4. Mixed-substrate experiments support the hypothesis that the enzyme responsible for the sulphation of l-tyrosine methyl ester and tyramine is separate from that responsible for the sulphation of p-nitrophenol. However, p-nitrophenol is a potent inhibitor of the sulphation of both tyrosyl derivatives whereas these latter compounds have no effect on the sulphation of p-nitrophenol.

/pdf/partial-purification-and-properties-of-an-enzyme-from-rat-118tp8bbw1.pdf

Partial purification and properties of an enzyme from rat liver that catalyses the sulphation of L-tyrosyl derivatives.

Kinetic studies of the phenol sulphotransferase reaction

Purification, Characterization, and Molecular Cloning of A Novel Rat Liver Dopa/Tyrosine Sulfotransferase

CHAPTER 8 – Sulfate Activation and Transfer

A sensitive method using the Technicon Amino Acid Autoanalyzer was developed for the determination of l-tyrosine O-sulphate in mammalian urine. Normal rabbit urine was shown to contain a material that has properties identical with l-tyrosine O-sulphate. The material is also a normal constituent of rat, sheep, calf and mouse urine and is excreted by these animals in quantities ranging from 0·1 to 0·8mg./day/kg. body wt.

The detection and determination of L-tyrosine O-sulphate in rabbit and other mammalian urine.

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The detection and determination of L-tyrosine O-sulphate in rabbit and other mammalian urine.