R. Glöckner
University of Jena
23 Papers
320 Citations
R. Glöckner is an academic researcher from University of Jena. The author has contributed to research in topics: Enzyme inducer & In vivo. The author has an hindex of 12, co-authored 23 publications.
Chat about Author
Papers
New hepatocyte in vitro systems for drug metabolism: metabolic capacity and recommendations for application in basic research and drug development, standard operation procedures.
Rolf Gebhardt,Jan G. Hengstler,Dieter Müller,R. Glöckner,Peter Buenning,Britta Laube,Eva Schmelzer,Martina Ullrich,Dietmar Utesch,Nicola J. Hewitt,M. Ringel,Beate Reder Hilz,Augustinus Bader,Angelika Langsch,Thomas Koose,Hans-Jörg Burger,Jochen Maas,Franz Oesch +17 more
TL;DR: Five in vitro systems with hepatocytes in suspension, perifusion culture systems, liver slices, co-culture systems of hepatocytes with intestinal bacteria, and 96-well plate bioreactors are discussed, which offer the possibility of high throughput and automatization.
275
Application of cryopreserved precision-cut liver slices in pharmacotoxicology--principles, literature data and own investigations with special reference to CYP1A1-mRNA induction.
TL;DR: The results derived from one sample of cryopreserved human liver slices indicate a quite acceptable maintenance of function up to 6 h, if the same protocol as developed for rat liver slices was used.
39
Monooxygenation, cytochrome P450-mRNA expression and other functions in precision-cut rat liver slices.
TL;DR: Precision-cut rat liver slices are a useful tool for the study of various hepatic functions, drug metabolism and its induction in vitro.
35
Induction of cytochrome P450 2B1-mRNA and pentoxyresorufin O-depentylation after exposure of precision-cut rat liver slices to phenobarbital.
TL;DR: Precision-cut rat liver slices proved to be a simple and reliable in vitro system for the sensitive detection of an induction by phenobarbital.
34
Use of Fresh and Cryopreserved Human Liver Slices in Toxicology with Special Reference to In vitro Induction of Cytochrome P450.
TL;DR: Even in thawed human slices, CYP1A1-mRNA expression could be induced in vitro by BNF, although at a very low level and preferentially in those specimens with comparably high inducibility already before freezing.
31