Statistical Methods in Medical Research.

A review of adhesion science.

Prevention of demineralization around orthodontic brackets in vitro.

STATEMENT OF PROBLEM: The effect of surface finish of dental materials on the subsequent contamination by microorganisms. PURPOSE: This study compared the retention of Streptococcus oralis, Actinomyces viscosus, and Candida albicans on polished, sandblasted (fine and coarse) and electrobrightened cobalt-chromium alloy and dental acrylic resin to assess in vitro the effect of such techniques on prosthesis contamination. MATERIAL AND METHODS: Standardized cell suspensions were incubated with test materials for 1 hour at 37 degrees C, after which retained cells were counted by using image analysis (percentage area of a microscopic field covered by cells). RESULTS: Retention of bacterial cells was substantial (S oralis 12% to 20% and A viscosus 9% to 16%) irrespective of surface finish. Maximal retention was observed on cobalt-chromium alloy that had undergone fine sandblasting and electrobrightening (P < .01). For C albicans, an increase in surface roughness (0.15 to 3.53 microns) resulted in an increase in retention (3% to 9%). CONCLUSION: Cell size and the type of roughening significantly affected the retention of microorganisms on surfaces. Electrobrightening of cobalt-chromium alloy did not reduce the surface roughness or subsequent cell attachment.

Retention of oral microorganisms on cobalt-chromium alloy and dental acrylic resin with different surface finishes.

A protocol for determining the surface free energy of dental materials

Surface characteristics that are considered important for bacterial attachment to thirteen orthodontic bonding composite cements and one glass ionomer cement were examined in vitro before and after toothbrush abrasion. The surface roughness and contact angle measurements were found to be statistically significantly different between the materials, both before and after brushing, and there were also statistically significant changes within materials after brushing. There were low correlation coefficients between surface roughness and contact angle for both pre-and post-brushed materials.

Surface roughness and droplet contact angle measurement of various orthodontic bonding cements.

An experimental technique was developed to examine the adhesion of a strain of Streptococcus mutans to the surface of discs of selected orthodontic bonding composites and a glass ionomer cement. Colonization by the bacterium was assessed by weight gain and scanning electron microscopy. Initial colonization occurred after about 3 days, and the specimens were usually completely covered by the fourth day. There were statistically significant differences between materials for weight gain, a strong correlation between weight gain and surface free energy of the materials measured as droplet contact angle (r = 0.9) and a moderate correlation between weight gain and surface roughness (r = 0.6).

Microbial Growth on the Surfaces of Various Orthodontic Bonding Cements

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Surface roughness and droplet contact angle measurement of various orthodontic bonding cements.

Microbial Growth on the Surfaces of Various Orthodontic Bonding Cements