Plant root exudates play important roles in the rhizosphere. We tested three media (nutrient solution, deionized water and CaSO4 solution) for three periods of time (2, 4 and 6 h) for collecting root exudates of soil-grown rice plants. Nutrient culture solution created complications in the analyses of exudates for total organic C (TOC) by the wet digestion method and of organic acids by HPLC due to the interference by its components. Deionized water excluded such interference in analytical analyses but affected the turgor of root cells; roots of four widely different rice cultivars excreted 20 to 60 % more TOC in deionized water than in 0.01 M CaSO4. Furthermore, the proportion of carbohydrates in TOC was also enhanced. Calcium sulfate solution maintained the osmotic environment for root cells and did not interfere in analytical procedures. Collection for 2 h avoided under-estimation of TOC and its components exuded by rice roots, which occurred during prolonged exposure. By placing plants in 0.01 M CaSO4 for 2 h, root exudates of soil-grown traditional, tall rice cultivars (Dular, B40 and Intan), high-yielding dwarf cultivars (IR72, IR52, IR64 and PSBRc 20), new plant type cultivars (IR65598 and IR65600) and a hybrid (Magat) were collected at seedling, panicle initiation, flowering and maturity and characterized for TOC and organic acids. The exudation rates were, in general, lowest at seedling stage, increased until flowering but decreased at maturity. Among organic acids, malic acid showed the highest concentration followed by tartaric, succinic, citric and lactic acids. With advancing plant growth, exudation of organic acids substituted exudation of sugars. Root and shoot biomass were positively correlated with carbon exudation suggesting that it is driven by plant biomass. As root exudates provide substrates for methanogenesis in rice fields, large variations in root exudation by cultivars and at different growth stages could greatly influence CH4 emissions. Therefore, the use of high-yielding cultivars with lowest root excretions, for example IR65598 and IR65600, would mediate low exudate-induced CH4 production. The screening of exciting rice cultivars and breeding of new cultivars with low exudation rates could offer an important option for mitigation of CH4 emission from rice agriculture to the atmosphere.

Characterization of Root Exudates at Different Growth Stages of Ten Rice (Oryza sativa L.) Cultivars

Optimization of the determination of organic acids and sugars in fruit juices by ion-exclusion liquid chromatography

Optimisation of an HPLC method for the simultaneous quantification of the major sugars and organic acids in grapevine berries.

Role of chromatography in the analysis of sugars, carboxylic acids and amino acids in food.

A method to simultaneously identify and quantitate the major carboxylic acids, sugars, glycerol, and ethanol in wines and grape musts is proposed. The technique involves isocratic separation, an ion-exchange column, and refractive index and UV detection (at 214 nm) without sample preparation. Statistical evaluation shows that the high-performance liquid chromatographic (HPLC) method is highly reproducible and reliable. The results of the HPLC analyses and the data obtained by standard methods are compared.

/pdf/simultaneous-determination-of-the-major-organic-acids-sugars-1x5pohepyy.pdf

Simultaneous Determination of the Major Organic Acids, Sugars, Glycerol, and Ethanol by HPLC in Grape Musts and White Wines

Improved high-performance liquid chromatographic analysis of some carboxylic acids in food and beverages as their p-nitrobenzyl esters

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Improved high-performance liquid chromatographic analysis of some carboxylic acids in food and beverages as their p-nitrobenzyl esters