Quido A. Valent
VU University Amsterdam
13 Papers
281 Citations
Quido A. Valent is an academic researcher from VU University Amsterdam. The author has contributed to research in topics: Signal recognition particle & Signal peptide. The author has an hindex of 12, co-authored 13 publications.
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Papers
The Escherichia coli SRP and SecB targeting pathways converge at the translocon
Quido A. Valent,Pier A. Scotti,Stephen High,Jan-Willem de Gier,Gunnar von Heijne,Georg Lentzen,Wolfgang Wintermeyer,Bauke Oudega,Joen Luirink +8 more
TL;DR: It is demonstrated here that the SRP pathway delivers nascent inner membrane proteins at the membrane through a protein cross‐linking approach that delivers a variety of precursor proteins to a common membrane translocation complex of the E. coli inner membrane.
Nascent membrane and presecretory proteins synthesized in Escherichia coli associate with signal recognition particle and trigger factor.
Quido A. Valent,Jan-Willem de Gier,Gunnar von Heijne,Debra A. Kendall,Corinne M. ten Hagen-Jongman,Bauke Oudega,Joen Luirink +6 more
TL;DR: Evidence is presented that the overproduction of proteins that expose hydrophobic polypeptide stretches, titrates SRP and trigger factor, indicating that inner membrane proteins are the primary physiological substrate of the E. coli SRP.
196
Assembly of a cytoplasmic membrane protein in Escherichia coli is dependent on the signal recognition particle
Jan-Willem de Gier,Parvaneh Mansournia,Quido A. Valent,Gregory J. Phillips,Joen Luirink,Gunnar von Heijne +5 more
TL;DR: Targeting of the cytoplasmic membrane protein leader peptidase (Lep) and a Lep mutant that inserts with an inverted topology compared to the wild‐type protein was studied in Escherichia coli strains that are conditional for the expression of either Ffh or 4.5S RNA, indicating that SRP is required for the assembly of cytopolasmic membranes proteins in E. coli.
172
Differential use of the signal recognition particle translocase targeting pathway for inner membrane protein assembly in Escherichia coli.
Jan-Willem de Gier,Pier A. Scotti,Annika Sääf,Quido A. Valent,Andreas Kuhn,Joen Luirink,Gunnar von Heijne +6 more
TL;DR: In this paper, the authors show that inner membrane proteins can assemble either by a mechanism in which SRP delivers the protein at the preprotein translocase or by what appears to be a direct integration into the lipid bilayer.
135
Differential use of the signal recognition particle translocase targeting pathway for inner membrane protein assembly in Escherichia coli (preprotein translocaseyprotein targeting)
Jan-Willem L. De Gier,Pier A. Scotti,Quido A. Valent,A Ndreas Kuhn,J Oen Luirink,Gunnar von Heijne +5 more
- 01 Jan 1998
TL;DR: Observations indicate that inner membrane proteins can assemble either by a mechanism in which SRP delivers the protein at the preprotein translocase or by what appears to be a direct integration into the lipid bilayer.
129