10 Papers
23 Citations
Qi Yan is an academic researcher from Carnegie Mellon University. The author has contributed to research in topics: Endocytosis & Exocytosis. The author has an hindex of 7, co-authored 10 publications. Previous affiliations of Qi Yan include University of New Mexico.
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Papers
Fluorogen-activating single-chain antibodies for imaging cell surface proteins
Christopher Szent-Gyorgyi,Brigitte F. Schmidt,Yehuda Creeger,Gregory W. Fisher,Kelly L Zakel,Sally A. Adler,James A. J. Fitzpatrick,Carol A. Woolford,Qi Yan,Kalin V. Vasilev,Peter B. Berget,Marcel P. Bruchez,Jonathan W. Jarvik,Alan S. Waggoner +13 more
TL;DR: The development of protein reporters that generate fluorescence from otherwise dark molecules (fluorogens) is reported, isolated by screening a library of human single-chain antibodies using derivatives of thiazole orange and malachite green.
STED Nanoscopy in Living Cells Using Fluorogen Activating Proteins
James A. J. Fitzpatrick,Qi Yan,Jochen J. Sieber,Marcus Dyba,Ulf Schwarz,Christopher Szent-Gyorgyi,Carol A. Woolford,Peter B. Berget,Alan S. Waggoner,Marcel P. Bruchez +9 more
TL;DR: It is demonstrated the effectiveness of a genetically encoded Malachite Green binding fluorogen activating protein for live cell stimulated emission depletion nanoscopy (STED) and suggests a resolution of 70nm with the given instrument.
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Localization Microscopy using Noncovalent Fluorogen Activation by Genetically Encoded Fluorogen‐Activating Proteins.
Qi Yan,Samantha L. Schwartz,Suvrajit Maji,Suvrajit Maji,Fang Huang,Chris Szent-Gyorgyi,Diane S. Lidke,Keith A. Lidke,Marcel P. Bruchez +8 more
TL;DR: The super-resolution properties and images obtained from a selected FAP clone fused to actin are evaluated, and it is shown that the photon counts per object are between those typically reported for fluorescent proteins and switching-dye pairs, resulting in 10-30 nm localization precision per object.
Near-instant surface-selective fluorogenic protein quantification using sulfonated triarylmethane dyes and fluorogen activating proteins
Qi Yan,Brigitte F. Schmidt,Lydia A. Perkins,Matharishwan Naganbabu,Saumya Saurabh,Susan Andreko,Marcel P. Bruchez +6 more
TL;DR: An improved labeling approach based on the genetic fusion of a fluorogen activating protein (FAP) to a GPCR and binding of a sulfonated analog of the malachite green (MG) fluorogen to rapidly and selectively label cell surface receptors is reported.
Fluorogen-activating proteins provide tunable labeling densities for tracking FcεRI independent of IgE.
Samantha L. Schwartz,Qi Yan,Qi Yan,Cheryl A. Telmer,Cheryl A. Telmer,Keith A. Lidke,Keith A. Lidke,Marcel P. Bruchez,Marcel P. Bruchez,Diane S. Lidke,Diane S. Lidke +10 more
TL;DR: The use of fluorogen-activating proteins (FAPs) for single particle tracking of FcεRI to investigate how receptor mobility is influenced after IgE-induced changes in mast cell behavior and new insights are provided into the relationship among F cεRI structure, activity, and mobility.
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