The use of N-acetylneuraminic acid, sialyl-lactose, and glyco-macropeptide by bifidobacteria and lactobacilli, and their growth-promoting effects on B. longum, B. breve, B. bifidum, and B. infantis were investigated. The data presented here suggest that fortification with N-acetylneuraminic acid-containing substances of infant formula may provide formula-fed infants with a function that human milk possesses.

Growth-promoting Effects of N-Acetylneuraminic Acid-containing Substances on Bifidobacteria

https://onlinelibrary.wiley.com/doi/pdf/10.1111/1471-0307.12555

Antimicrobial and antidiabetic potential of synbiotic fermented milk: A functional dairy product

Improved viability of Lactobacillus plantarum embedded in whey protein concentrate/pullulan/trehalose hydrogel during freeze drying.

In the present study, conditions for Bacillus mucilaginous fermentation using Agaricus bisporus wastewater as culture medium were optimized. We analyzed the total number of living B. mucilaginous in the fermentation broth using multispectral imaging flow cytometry. Single-factor experiments were carried out, where a Plackett–Burman design was used to screen out three factors from the original six factors of processing wastewater solubility, initial pH, inoculum size, liquid volume, culture temperature, and rotation speed that affected the total number of viable B. mucilaginous. The Box–Behnken response surface method was used to optimize interactions between the three main factors and predict optimal fermentation conditions. Factors significantly affecting the total number of viable B. mucilaginous, including shaking speed, culturing temperature, and initial pH, were investigated. The optimum conditions for B. mucilaginous fermentation in A. bisporus wastewater were a rotational speed of 195 rpm, culture temperature of 29 °C, initial pH of 6.5, solubility of 0.5%, 8% inoculation volume, and 90 mL liquid volume in a 250 mL flask, culture time of 48 h. Under these conditions, the concentration of total viable bacteria reached 2.16 ± 0.02 × 108 Obj/mL, which meets the national standard. A. bisporus wastewater can be used for the cultivation of B. mucilaginous.

/pdf/optimization-of-the-culture-condition-of-bacillus-22vx4xe27r.pdf

Optimization of the culture condition of Bacillus mucilaginous using Agaricus bisporus industrial wastewater by Plackett–Burman combined with Box–Behnken response surface method

The present invention relates to a cryoprotecting agent comprising a cryprotectant being one or more of: dextrin, dextran, isomaltooligosaccharide and derivatives thereof, cryoprotecting and cryopreserved compositions, uses thereof, and methods of cryopreservation.

Cryoprotecting agent, cryoprotecting and cryopreserved compositions, uses thereof, and methods of cryopreservation

The invention discloses a preparation method of synbiotic goat milk powder, which comprises the following steps: activating probiotics to obtain probiotic mire; adding a compound freeze-drying protective agent; obtaining freeze-drying probiotic powder by freeze-drying; carrying out purification, standardization and reduced pressure condensation on a raw material of goat milk to obtain new raw material of goat milk; adding prebiotics in the condensed raw material of goat milk and then carrying out spray drying to obtain goat milk powder containing the prebiotics; cooling the goat milk powder to room temperature and filtering with a screen; and mixing the screened goat milk powder containing the prebiotics with the probiotic powder under the aseptic condition to obtain the symbiotic goat milk powder. In the invention, the prebiotics are added into condensed degrease goat milk by condensation or whole goat milk and are beneficial to the uniform dispersion of the prebiotics; the probiotics are prepared by a freeze-drying method and added with the freeze-drying protective agent, so that the viable bacteria is more and bacteria has a colony of over 1,010cfu/g; and by adding the prebiotics into the goat milk powder, the prebiotics can be protected and the survival rate of the prebiotics and the functionality of the goat milk powder can be improved.

Preparation method of synbiotic goat milk powder

Four different prebiotics including fructo-oligosaccharide isomalto-oligosaccharide, inulin and xylo-oligosaccharide were screened for their effects on survival of Bifidobacterium bifidum during freeze-drying. The additions of prebiotics (v/v) were all 4%, 8%, 12%, 16% and 20%. The results showed that prebiotics could act on Bifidobacterium Bifidum. The optimum concentration of fructo-oligosaccharides (FOS) in samples for surival of Bifidobacterium bifidum was 12% and for viable count was 20％. Addition of xylo-oligosaccharides (XOS) played a significant role in promoting cell viablity when it was in low level. The optimum concentration of isomalto-oligosaccharides (IMO) for surival of Bifidobacterium bifidum was 16% and for viable count was 12％, while inulin in samples for surival and viable cell of Bifidobacterium bifidum both was 4％.

Effect of Fructo-Oligosaccharide, Isomalto-Oligosaccharide, Inulin and Xylo-Oligosaccharide on Survival of B. Bifidum during Freeze-Drying

The invention disclosed synbiotic goat milk powder, which comprises the following components in part by mass: 940-980 parts of goat milk powder, 10-50 parts of prebiotics and 1.0-10 parts of probiotics. In the invention, the prebiotics and the probiotics are added in the goat milk powder, wherein the prebiotics can relieve the constipation, reduce the pH value of intestines, recover the intestinal bacterium balance, has the efficacy of reducing blood fat, reduces the morbidity of rectal cancer, completes baby intestinal bacterial colony and promotes the absorption of mineral substances such as Ca and Mg ; the probiotics have remarkable facilitation on human body health, can increase the nutritional value, treat diarrhea and turista which are caused by rotavirus and diarrhea caused by antibiotic and reduce the content of serum cholesterol. The invention has the advantages of relieving the lactose intolerance, allergic dermatitis, irritable bowel syndrome and inflammatory bowel disease, treating vaginalitis caused by candida and bacteria, adjusting the immune function of human body and the intestinal microecology balance and improving the constipation. In addition, the invention has beneficial effects on surface bladder cancer and cervical carcinoma and the functions of resisting mutagenesis or carcinogenic activity and tumor.

Synbiotic goat milk powder

The invention discloses a synbiotics goat milk slice and a method for preparing the same. The method comprises the following steps of: firstly, inoculating activated probiotics to the fermentation tank of an MRS culture medium for cultivation, performing centrifuging to prepare probiotics mud, adding a composite freeze-drying protective agent to the probiotics mud and carrying out vacuum freeze-drying to obtain freeze-dried probiotics powder; carrying out decompression concentration, spray drying and cooling till the temperature reaches the room temperature after goat milk is purified and standardized to obtain goat milk powder; evenly mixing the goat milk powder, the probiotics, cane sugar, glucose, synbiotics and magnesium stearate, and then preparing the synbiotics goat milk slices by means of a single-punch tablet press. The synbiotics goat milk slice is added with the probiotics and the synbiotics; therefore, the physiological bacterial activity of the probiotics can be brought into play; and the quantity of the bacteria can be quickly increased selectively, so that the effect of the probiotics is more obvious and longer; and the probiotics and the synbiotics are combined to resist diseases together and to maintain micro-ecological balance of the organism.

Synbiotics goat milk slices and method for preparing same

Effect of four materials including trehalose, soluble starch, raffinose and galactose on survival of Lactobacillus acidophilus was studied by counting viable cells before and after freeze-drying. The concentrations of five carbohydrates were all 3%, 6%, 9%, 12% and 15%. Results showed as follows: the optimal concentrations of trehalose, soluble starch, raffinose and galactose were 12%, 9%, 3% and 15%, the survival of Lactobacillus acidophilus was 42.62%, 56.50%, 45.05% and 42.69% and the viable cells of lyophilized powder were 0.40×1011cfu/g,0.45×1011cfu/g, 0.52×1011 and 1.09×1011cfu/g, respectively.

Effect of Four Materials Including Trehalose, Soluble Starch, Raffinose and Galactose on Survival of Lactobacillus acidophilus during Freeze-Drying

Handle everyday research tasks with reliable, citation-backed results

Your personal Research Agent to handle research tasks with citation-backed results

Popular Tasks used by Researchers

How can I help with your research?

Meet SciSpace

Get more enhanced response by uploading the PDFs you want me to reference.

No relevant PDFs in your library

SciSpace is the AI research assistant for academics. Run systematic literature reviews on 280M+ papers, and write papers with cited sources. Trusted by 1M+ students, PhDs & researchers.

SciSpace | AI for Research

Analyze PDFs

Code & Manuscripts

Funding & Grants

Literature & Patents

Medical & Clinical Data

Systematic Review

Visualize & Present

Web & Data

Build a Google Scholar-like website for your research.

Build a website

Create charts and images for your research

Create a Chart

Write a paper for submission to a journal

Draft a manuscript

Patent Search

Design eye-catching scientific posters in minutes.

Scientific Poster Generation

Systematic Literature Review

One task is running at the moment. Your messages will be shown right after.

Drag and drop or click here to browse

Loved by <highlight>1 million+</highlight> researchers

Extract a list of specific topics and their sources from unstructured text

Topics

Compare and analyze relevant papers that matches with your search

Papers

Get insights from PDFs and bookmarked papers from your library

My library

Recent searches

Try searching for:

Catch AI-generated content in scholarly and non-scholarly content

{ai} Detector

Ai Writer

Get PDF Summaries, highlighted text explanations 

Chat with PDF

Effortlessly create in-text citations and bibliographies in APA and 2,500 other formats

Citation generator

Get explanations, summaries, and answers on academic papers

Ease up your research workflow with {scispace}'s cohort of exciting AI tools

Elevate your academic writing skills and convey your ideas the way you want

Paraphraser

Explore our range of reading and writing tools

Your file is being prepared and should be ready in a few minutes. If it's a large file, it might take a bit longer. You can close this window, and we'll email you the file when it's done.

You have reached a maximum limit of <strong>{limit}</strong> columns in the table. Remove at least <strong>1</strong> column to add or create another one.

Qi Ma

Author Tools

Chat about Author